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Issue:ISSN 1000-7083
          CN 51-1193/Q
Director:Sichuan Association for Science and Technology
Sponsored by:Sichuan Society of Zoologists; Chengdu Giant Panda Breeding Research Foundation; Sichuan Association of Wildlife Conservation; Sichuan University
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Your Position :Home->Past Journals Catalog->2013 Vol.32 No.3

In Vitro Effects of Heavy Metal on Cell Viability of Haemocyte from Abalone Haliotis diversicolor
Author of the article:XIAN Jian’an,MIAO Yutao,PAN Xunbin,WANG Anli *
Author's Workplace:(Key Laboratory of Ecology and Environment Science in Guangdong Higher Education, Guangdong Provincial Key Laboratory for Healthy and Safe Aquaculture, School of Life Science, South China Normal University, Guangzhou 510631, China)
Key Words:Haliotis diversicolor; haemocyte; cell viability; flow cytometry
Abstract:After in vitro exposure with 10-3~10-9 M heavy metals (Cd2+, Hg2+, Cu2+ and Zn2+), cell viability of haemocytes from Haliotis diversicolor were determined using PI staining by flow cytometry. Results showed that after in vitro exposure for 6 h, 10-3M and 10-4M Cd2+, 10-3M, 10-4M and 10-5M Hg2+, 10-3M Cu2+, 10-3M and 10-4M Zn2+ significantly decreased the cell viability (P<0.05). Mortality of H. diversicolor haemocytes were 41.76%, 48.75%, 23.28% and 28.52% after exposed to 10-3M Cd2+, Hg2+, Cu2+ and Zn2+ respectively. The toxicity of these four heavy metals on H. diversicolor haemocytes can be ranked in the following order: Hg2+ >Cd2+> Zn2+> Cu2+.
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