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Issue:ISSN 1000-7083
          CN 51-1193/Q
Director:Sichuan Association for Science and Technology
Sponsored by:Sichuan Society of Zoologists; Chengdu Giant Panda Breeding Research Foundation; Sichuan Association of Wildlife Conservation; Sichuan University
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Your Position :Home->Past Journals Catalog->2013 Vol.32 No.2

Divergence of Plague Resistance between Lasiopodomys brandtii and Lasiopodomys fuseus in Natural Populations
Author of the article:WEI Lei1, 2, WANG Chengmin1, HU Yi1, LUO Jing1, ZHANG Min1, HE Hongxuan1?
Author's Workplace:(1. National Research Center for Wildlife Born Diseases, Key Laboratory of Animal Ecology and Conservation Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China; 2. Faculty of Animal Science, Suzhou Vocational Technology College, Suzhou, Anhui Province 234101, China)
Key Words:MHC class Ⅱ gene β; plague; resistance; susceptibility
Abstract:Objective  MHC class Ⅱ gene β was used as candidate gene to investigate the genetic basis of plague resistance in natural populations of Lasiopodomys brandtii and L. fuseus experimentally infected with virulent Y. pestis. Methods  We compared allelic frequencies and genotypes frequencies of RFLPs-RsaMHC class Ⅱ gene β of dying and viable plague challenged L. brandtii and L. fuseus. Rusults  Our results suggested that allele RsaE and RsaF were associated with resistance to plague in L. brandtii (resistant individuals: 0.3095, 0.2738; susceptible individuals: 0.0571, 0.0286; P0.01); allele RsaF was associated with resistance to plague in L. fuseus (resistant individuals: 0.3704; susceptible individuals: 0.0682; P0.01). Sequence analysis showed that 68 bases mutations were found, 39 bases mutations in L. brandtii and 27 bases mutations in L. fuseus. Statistical results showed that polymorphism sites at positions 27, 53, 63, 70, 91, 126, 127, 128, 129, 160, 213, 214, 215 in L. brandtii and 27, 53, 63, 126, 127, 128, 129, 160, 213, 214, 215 in L. fuseus. RsaE alleles were not discovered in L. fuseus by comparing with sequences of MHC class Ⅱ gene β of L. brandtii. This may reveal the molecular mechanisms for divergence of plague resistance between L. brandtii and L. fuseus in natural populations. Conclusion  The polymorphism of MHC class Ⅱ gene β may involved in L. brandtii and L. fuseus plague resistance. MHC class Ⅱ gene β and other undetermined plague resistance markers would provide useful biological information about host evolution and disease dynamics.
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