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Issue:ISSN 1000-7083
          CN 51-1193/Q
Director:Sichuan Association for Science and Technology
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Your Position :Home->Past Journals Catalog->2012 Vol.31 No.6

Effect of Toxoplasma gondii RH Strain on Cell Cycle of Mouse Placental Trophoblasts
Author of the article:GE Pu1,2, ZHENG Wei-ping 3 , LI Chun-li4 , LIU Yu-jiao5 , YE Bin2,6*
Author's Workplace:( 1. Department of Pathophysiology, Chongqing Medical University, Chongqing 400016, China; 2. Center for Tumor and Molecular Medical Experiment, Chongqing Medical University, Chongqing 400016, China; 3. Functional laboratory of Experimental Center, Chongqing Medical University, Chongqing 400016, China; 4. Department of Life Science, Chongqing Medical University, Chongqing 400016, China; 5. Department of Pediatrics of Seven School System, Chongqing Medical University, Chongqing 400016, China;6. Department of Pathobiology, Chongqing Medical University, Chongqing 400016, China)
Key Words: Toxoplasma gondii RH strain; trophoblastic cells; cell cycle
Abstract:Objective  To investigate the effects of Toxoplasma gondii RH strain on cell cycle of mouse placental trophoblastic cells in vitro. Methods  Mouse placental trophoblastic cells (concentration of 5×104/mL) were harvested in the different cell culture plates. The cells were treated for different durations (6~24 h) with different concentrations of T. gondii RH strain (the concentration of tachyzoites was 2×104/mL, 4×104 /mL, 8×104/mL, respectively). Growth inhibition rates were measured with the MTT methodCell cycle was examined with flow cytometer. Western blot was used to detect the levels of cyclinA, cdk2 of cells. Results  T. gondii RH strain could inhibit the proliferation of trophoblastic cells in a time-dose dependent manner. Cell cycle was significantly stopped at G2/M phase by T. gondii RH strain with FCM technology. Infection of T. gondii RH strain reduced the expressions of gene cyclinA and cdk2 of trophoblastic cells. Conclusion  Infection of T. gondii RH Strain could inhibit trophoblastic cells and arrest the cell cycle mainly by regulating the expression of gene cyclinA and cdk2.
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