Construction of Porcine Defensin PD Gene Expression Vector
Author of the article:LUO Gang, WEI Hong
Author's Workplace：（Laboratory Animal Center, Third Military Medical University, Chongqing 400038, China）
Key Words：native antimicrobial peptide; porcine defensin PD gene; expression vector
Abstract：Objective To construct the orcine defensin PD gene expression vector. Method The PD gene transformed was synthesized chemically and inserted into fusion expression vector PinPoint TMXa-3. Result The result showed that the orcine PD gene expression vector was established successfully with restriction enzyme analysis and DNA sequencing. Conclusion The porcine PD gene expression vector was successfully constructed, which lays down the foundation in research of antimicrobial activities, antimicrobial mechanism of the native antimicrobial peptide.