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Issue:ISSN 1000-7083
          CN 51-1193/Q
Director:Sichuan Association for Science and Technology
Sponsored by:Sichuan Society of Zoologists; Chengdu Giant Panda Breeding Research Foundation; Sichuan Association of Wildlife Conservation; Sichuan University
Address:College of Life Sciences, Sichuan University, No.29, Wangjiang Road, Chengdu, Sichuan Province, 610064, China
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Your Position :Home->Past Journals Catalog->2012 Vol.31 No.5

Microsatellite Enrichment by Magnetic Beads in Cervus nippon sinchuanicus
Author of the article:LIU Meng-yao1#, YANG Cheng-zhong1#, GAO Yi-min2, XIAO Zhen3, WANG Lin-bo2, WANG Jie-qing2, LIU Wu-hu
Author's Workplace:(1. Sichuan Key Laboratory of Conservation Biology on Endangered Wildlife, College of Life Sciences, Sichuan University, Chengdu 610064, China; 2. Jiangxi Taohongling Sika Deer National Nature Reserve, Pengze,Jiangxi Province 332700, China; 3. Jiangxi Xingan Middle School, Ji’an, Jiangxi Province 331300, China)
Key Words: Cervus nippon sinchuanicus; magnetic beads adsorption method; microsatellite
Abstract:

The microsatellite loci from Cervus nippon sinchuanicus genome was isolated by Streptavidin-coated Magnetic Beads Adsorption method. The length of 200~750 bp DNA fractions containing microsatellite sequences were captured by oligonucleotide probes. The enriched DNA segments were ligated on pMD18-T and then transformed into E. coli JM109 competent cells. Then PCR was applied to screen positive clones from the libraries. All 234 positive clones were identified from 576 transformants. As a result, 73 sequences were acquired. Besides (CAG)n, (AG)and (AT)n repeat sequences were also obtained. The results indicated that the method was efficient to isolate microsatellite markers. The markers will be useful tools for further studies on C. n. sinchuanicus genetics and genomics.

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