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Issue:ISSN 1000-7083
          CN 51-1193/Q
Director:Sichuan Association for Science and Technology
Sponsored by:Sichuan Society of Zoologists; Chengdu Giant Panda Breeding Research Foundation; Sichuan Association of Wildlife Conservation; Sichuan University
Address:College of Life Sciences, Sichuan University, No.29, Wangjiang Road, Chengdu, Sichuan Province, 610064, China
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Your Position :Home->Past Journals Catalog->2012 Vol.31 No.5

Transcriptional Expression of MBD1 Gene and Its Regulation by DNA Methylation in Bovine Tissues
Author of the article:WANG Hui-min, AO Xu-dong, YUE Yong-li, GAO Hai-xia, YU Hai-quan*
Author's Workplace:(The Key Laboratory of Mammal Reproductive Biology and Biotechnology, Ministry of Education, Inner Mongolia University, Hohhot 010021, China)
Key Words:MBD1; tissue; DNA methylation; mRNA expression
Abstract:

DNA methylation is the main epigenetic regulation which regulate gene expression at transcriptional level. Methyl-CpG binding protein MBD1 specially recognized methylated as well as unmethylated DNA through its transcription repression domains to affect gene expression, MBD1 play an important role between DNA methylation and transcriptional repression, but the regulation of DNA methylation on MBD1 gene expression remained unclear. In this study, transcriptional expression of MBD1 was analyzed by RT-PCR in bovine heart, liver, kidney, testis, and ovary. Twelve CpG sites in the regulation region of MBD1 were chosen and their DNA methylation status were checked by bisulfite-sequencing PCR method, the dynamic change of DNA methylation status in the regulatory region of MBD1 were also examined in bovine tissues. Results shows that the transcriptional level of MBD1 gene were lower in heart and kidney than of the liver, testis and ovary, the differences was significantly (P<0.05); the DNA methylation status of MBD1 regulatory region was also lower in heart and kidney than that of liver, testis and ovary, which suggested that the expression of bovine MBD1 is associated with the DNA methylation of its regulation region.

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