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Issue:ISSN 1000-7083
          CN 51-1193/Q
Director:Sichuan Association for Science and Technology
Sponsored by:Sichuan Society of Zoologists; Chengdu Giant Panda Breeding Research Foundation; Sichuan Association of Wildlife Conservation; Sichuan University
Address:College of Life Sciences, Sichuan University, No.29, Wangjiang Road, Chengdu, Sichuan Province, 610064, China
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Your Position :Home->Past Journals Catalog->2012 Vol.31 No.5

Cloning, Sequence Analysis and Expression of the Giant Panda FOXL2 Gene
Author of the article:  YUAN Hong-ying, YANG Dong, WANG Gao-chao, TAN Shuai, ZOU Fang-dong*
Author's Workplace:(Key Laboratory of Bio-resources and Eco-environment, College of Life Sciences, Sichuan University, Chengdu 610064, China)
Key Words:giant panda; FOXL2; gene cloning and expression; sequences analysis
Abstract:

FOXL2 is a member of the large family of winged helix/forkhead transcription factors involved in ovarian development and function. Herein we cloned the FOXL2 from the giant panda genome. The FOXL2 was sequenced, analyzed and expressed in E .coli BL21 and in HEK293 cells. The ORF of FOXL2 was inserted into prokaryotic expression vector pET-32a (+) to constructed recombinant expression plasmid pET-32a(+)-FOXL2 and expressed in E.coli BL21 by IPTG induction. A recombinant eukaryotic expression vector FOXL2-pcDNA3.1/V5-His C was constructed, the recombinant plasmid was transfected into HEK293 cells with Lipofectamine 2000 and the fusion protein was identified by Western blot analysis. SDS-PAGE and Western blot analysis showed that the recombinant FOXL2 was highly expressed at 4 hours after IPTG induction and the expressed product of FOXL2 was about 58.9 kDa which was recognized by His monoclonal antibody. The results could be useful for further study of the bioactivity and application of FOXL2.

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