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Issue:ISSN 1000-7083
          CN 51-1193/Q
Director:Sichuan Association for Science and Technology
Sponsored by:Sichuan Society of Zoologists; Chengdu Giant Panda Breeding Research Foundation; Sichuan Association of Wildlife Conservation; Sichuan University
Address:College of Life Sciences, Sichuan University, No.29, Wangjiang Road, Chengdu, Sichuan Province, 610064, China
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Your Position :Home->Past Journals Catalog->2012 Vol.31 No.4

Improvement of Growth And Immunity of Mice by Truncated Porcine IGF-1 Gene Encapsulated in Chitosan Nanoparticles
Author of the article:JIA Qian-qian1#, LI Dong1#, WAN Xiao-ping1#, YANG Xiao1, LI Ying2*, GAO Rong1*
Author's Workplace:(1. Key Laboratory of Bio-resources and Eco-environment, Ministry of Education, College of Life Sciences, Sichuan University, Chengdu 610064, China; 2. College of Chemistry, Sichuan University, Chengdu 610064, China)
Key Words:truncated porcine IGF-1 gene; growth; immunity; mice; chitosan nanoparticles
Abstract:Objective  To compare of effect of porcine IGF-1 and modified IGF-1 gene on the growth and immunity of mice, and develop safe and effective molecular regulator for immunity and growth of animal. Methods  The novel truncated porcine IGF-1 genes were cloned with overlapping PCR extension technique, and then respectively subcloned into prokaryotic and eukaryotic expression plasmid, pGEX-4T-1and VR1020, respectively designed as recombinant pGG, pGF, VRG and VRF. They were respectively expressed in vitro to evaluate their bioactivity, and packed with chitosan (CS) and mPEG-PEI derived CS prepared by ionotropic gelation method. They were then utilized to inoculate 3-week-old Kunming mice intramuscularly at the dose of 0.1 mg/per mouse, respectively. The blood were collected from the mice before and after inoculation on 0, 2, 4, 6, 7 and 8 weeks to detect the changes of weight, immune cells, CD4+/CD8+ T cells by FCM and influence on L-02 cell proliferation by MTT.  Results   It wasfound that the lymphocytes significantly increased in the blood of VRI and VRG groups from 14 to 42 days post inoculation compared with that of control mice, and the number of CD4+ T cells was also remarkably higher than the control (P<0.05). The growth performance of VRI and VRG groups were obviously better than the control group from the 14th day to 56th day after inoculation (P<0.05). The sera from mPEG-PEI-CS trapped VRI/VRG and CS-VRI/VRG could significantly promote the proliferation of L-02 cells. Conclusion   Our results firstly proved that VRG/F could markedly accelerate proliferation of L-02 cell from 14 to 42 days after inoculation in comparison with that of the control (P<0.05). These suggested that the growth and immunity of inoculated mice could be promoted by the truncated IGF-1 gene, which would inspire the development of safe and effective adjuvant to control of infectious diseases of animal in the future.
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