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Issue:ISSN 1000-7083
          CN 51-1193/Q
Director:Sichuan Association for Science and Technology
Sponsored by:Sichuan Society of Zoologists; Chengdu Giant Panda Breeding Research Foundation; Sichuan Association of Wildlife Conservation; Sichuan University
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Your Position :Home->Past Journals Catalog->2012 Vol.31 No.4

Molecular Identification of Falco cherrug Based on Cyt b and COI Gene
Author of the article:GUAN Jin-ke1, LI Yan-hong1, HU Jie1*, YANG Zhi-song1, YAO Gang1, WANG Min1,2
Author's Workplace: (1. College of Life Sciences, China West Normal University, Nanchong, Sichuan Province 637009, China; 2. Zigong No.6 High School, Zigong, Sichuan Province 643000, China)
Key Words:Falco cherrug; cytochrome b gene; cytochrome c oxidase subunit 1 gene; phylogenetic analysis; molecular identification
Abstract:In order to ensure the accuracy of wildlife law enforcement, the molecular identification was implemented for a Falcon bird in this study. In all, cytochrome b and cytochrome oxidase subunit 1 gene were amplified by PCR method and sequenced directly. Sequences of 8 species within Falco distributed in China, downloaded from GenBank, were compared with that of the sample, and the phylogenetic tree were constructed by the methods of maximum parsimony (MP) and Bayesian inference (BI). The results showed that sequence similarity between sample and Falco cherrug were 98.91%~99.41% in cytochrome b gene and 99.67%~100% in cytochrome oxidase subunit 1 gene. The genetic distance between sample and F. cherrug were 0.003~0.007 in cytochrome b gene and 0.000~0.003 in cytochrome oxidase subunit 1 gene. Furthermore, the suspected sample was clustered with the F. cherrug consistently both in the maximum parsimony tree and in the Bayesian tree. Consequently, it convincingly demonstrated that the sample was F. cherrug, which provided a powerful evidence for the trail of the case.
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