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Issue:ISSN 1000-7083
          CN 51-1193/Q
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Your Position :Home->Past Journals Catalog->2012 Vol.31 No.1

Study on Three-dimensional Culture and Adipogenic Differentiation of MSCs in Self-assembling Peptide Nanofiber Scaffolds
Author of the article:LIN Juan1, ZHOU Qing-han2, YANG Yu-han1*
Author's Workplace:(1. The School of Biomedical Science, Chengdu Medical College, Chengdu 610083, China; 2. College of Chemistry and Environment Protection Engineering, Southwest University for Nationalities, Chengdu 610041, China)
Key Words: self-assembling peptide; mesenchymal stem cells; three-dimensional culture; differentiation
Abstract:To investigate the adhesion, morphology and differentiation of MSCs in self-assembling peptide RADA16 hydrogel scaffolds, the 3rd passage of MSCs was seeded on RADA16 hydrogel for 3D culture. The morphology and survival of MSCs were observed by inverted phase contrast microscope and fluorescence staining. In the experimental group, adipogenic differentiation was achieved by the induction media. The control MSCs were maintained in DMEM media. Fluorescence staining showed that vast majority of MSCs in the RADA16 hydrogel could adhesive, survive, and distribute uniformly on different planes. After 7 d adipogenic induction intra-cellular lipid droplet appeared, and Oil Red O stained positive cells were identified. Differentiation was further demonstrated by Western blot analysis of adipocytic markers PPARγ expression. The results suggested that self-assembling peptide RADA16 hydrogel scaffolds offered suitable 3D micro-environment for the adhesion, growth, and differentiation of MSCs.
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