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Issue:ISSN 1000-7083
          CN 51-1193/Q
Director:Sichuan Association for Science and Technology
Sponsored by:Sichuan Society of Zoologists; Chengdu Giant Panda Breeding Research Foundation; Sichuan Association of Wildlife Conservation; Sichuan University
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Your Position :Home->Past Journals Catalog->2012 Vol.31 No.1

Metallothionein in Different Tissues of Meretrix meretrix Exposed to Zinc
Author of the article:WU Chao, CHEN Lu-xi, NI Xiao-ying, YING Xue-ping*
Author's Workplace:(School of Life and Environmental Sciences, Wenzhou University, Wenzhou, Zhejiang Province 325035, China)
Key Words:Meretrix meretrix; zinc; metallothionein; tissues
Abstract:In order to investigate the relationship between metallothionein (MT) content in foot, hepatopancreas, mantle, gill of Meretrix meretrix and the concentrations of zinc pollution, clam samples were exposed to different concentrations of zinc (0 mg/L, 1.5 mg/L, 3 mg/L, 6 mg/L) for a duration of 2 or 4 days. The zinc binding metallothionein was purified by the Bio-GelP-10Gel chromatography column, and was tested by UV spectrophotometer. The results showed that there was a great variation in MT expressed in the different tissues of M. meretrix after exposed to the same concentration of zinc, and the general order was foothepatopancreasmantlegills. The expression of MT presents a great variation in different tissues of M. meretrix after exposing the clams to different concentrations of zinc. General trend was that the MT content in different tissues decreased at first then increased over times. This research indicates that the content of MT may be affected by zinc exposure and that MT contents are different in various tissues to different concentrations of zinc for different times.
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