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Issue:ISSN 1000-7083
          CN 51-1193/Q
Director:Sichuan Association for Science and Technology
Sponsored by:Sichuan Society of Zoologists; Chengdu Giant Panda Breeding Research Foundation; Sichuan Association of Wildlife Conservation; Sichuan University
Address:College of Life Sciences, Sichuan University, No.29, Wangjiang Road, Chengdu, Sichuan Province, 610064, China
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Your Position :Home->Past Journals Catalog->2012 Vol.31 No.1

Comparison of the Fatty Liver and Insulin Resistance Animal Model in HFJ Inbred Rats and Wistar Closed Colony Rats
Author of the article:ZHENG Long, LIAN Wei-guang, LI Jian-hui, ZHANG Huan-ling, WANG Jun-xia, LIU Jun-xu, CAI Yue-hua, LIU
Author's Workplace:(Hebei Medical University, Hebei Key Lab of Laboratory Animal Science, Shijiazhuang 050017, China)
Key Words:HFJ inbred rat; Wistar closed colony rat; insulin resistance; fatty liver; animal model
Abstract:Objective  To establish fatty liver and insulin resistance animal model in HFJ inbred rats and Wistar closed colony rats, and compare their biological characteristics. Methods  Male HFJ rats and Wistar rats were randomly divided into model group and control group, which were fed with high-fat diet (44.2% of energy from fat) and standard diet (19.2% of energy from fat) respectively for 12 weeks. All rats were weighed every week. Fasting blood glucose, alanine aminotransferase (ALT), aspartate aminotransferase (AST), triglyceride (TG), total cholesterol (TC), HDLC, LDLC and fast insulin were tested by drawing blood from left ventricular. Liver wet weights were measured by freeing liver, and calculated liver index. Rat liver was fixed with 10% formalin and embedded with paraffin. Assessment of hepatic steatosis and inflammation in all rats is measured under light microscope by HE stain. Results  Liver tissue pathology showed that the hepatic cells of model group of HFJ and Wistar rats presented diffused macrovesicular steatosis and lobular inflammatory cell infiltration, and HFJ rats were more serious than Wistar rats. Rats in control group showed no abnormal. All rats in model group had elevated levels of AST, ALT, liver index and HOMA-IR, and there was no significant difference between HFJ rats and Wistar rats. Body weigh of the model group of HFJ rats were higher than those in control group (P<0.01), and there were no significant differences between model group and control group of Wistar rats(P>0.05). Serum TG and TC of the HFJ rats were obviously higher than that of Wistar rats. Conclusion  A fatty liver and insulin resistance model was successfully developed in HFJ inbred rats and Wistar rats by high-fat diet method. Compared with Wistar rats, HFJ rats had spontaneously high blood lipid characteristics, which provided a useful experimental tool for elucidating pathogenesis and treatment of fatty liver.
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