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Issue:ISSN 1000-7083
          CN 51-1193/Q
Director:Sichuan Association for Science and Technology
Sponsored by:Sichuan Society of Zoologists; Chengdu Giant Panda Breeding Research Foundation; Sichuan Association of Wildlife Conservation; Sichuan University
Address:College of Life Sciences, Sichuan University, No.29, Wangjiang Road, Chengdu, Sichuan Province, 610064, China
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Your Position :Home->Past Journals Catalog->2012 Vol.31 No.1

Study on the Method of Chromosome Preparation of Fig-pollinating Wasps (Hymenoptera, Agaonidae)
Author of the article:LIU Qing1,2, OU Xiao-hong3, HOU Xiang-lian1,2, YANG Da-rong1*
Author's Workplace:(1. Key Laboratory of Tropical Forest Ecology, Xishuangbannan Tropical Botanical Garden, Chinese Academy of Sciences, Kunming 650223, China; 2. Graduate University of Chinese Academy of Sciences, Beijing 100049, China; 3. Key Laboratory of Forest Disaster Warning and Control, Southwest Forestry University, Kunming 650224, China)
Key Words:fig-pollinating wasps; chromosome preparation; method
Abstract:The association between Ficus species and their pollinator-fig wasp is one of the most remarkable example of a plant–insect mutualism. The fig wasps need to lay their eggs in the minor receptive flowers of fig syconia and complete their lifecycle in it while they are pollinating the figs. With the materials of two fig-pollinating wasps species, Ceratosolen solmsi (pollinator of Ficus hispida, Hymenoptera, Agaonidae) and C. gravelyi (pollinator of F. semicordata, Hymenoptera, Agaonidae), a set of effective research method with detailed procedures of chromosome preparation is found. According to the method described here, the karyotype of C. solmsi and C. gravelyi are analysed, indicating that the two species have similar karyotype with a chromosome number of 2n=10(♀), all the chromosome are metacentric(NF=20) with little differences in relative length. Moreover, we compared this method with the one proposed by Imai et al. in 1988 to conclude that the procedure of this method described here is simplified and repeatable, with which a number of well defined and dispersed metaphase plates can be obtained.
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