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Issue:ISSN 1000-7083
          CN 51-1193/Q
Director:Sichuan Association for Science and Technology
Sponsored by:Sichuan Society of Zoologists; Chengdu Giant Panda Breeding Research Foundation; Sichuan Association of Wildlife Conservation; Sichuan University
Address:College of Life Sciences, Sichuan University, No.29, Wangjiang Road, Chengdu, Sichuan Province, 610064, China
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Your Position :Home->Past Journals Catalog->2006 Vol.25 No.1

Expression of Grass Carp Growth Hormone in Pichia pastoris
Author of the article:MENG Ya-peng1, XU Min1, DAI Huan-mei1, DING Xiu-qiong1, ZHANG Hai-ying1, YIN Xue1, LIU Shi-gui1,2*,
Author's Workplace:(1. College of Life Sciences, Sichuan University, Chengdu 610064; 2. Institute of Chengdu Chuangxin Biotechnology, Sichuan University)
Key Words:grass carp; growth hormone gene; Pichia pastoris; yeast; expression
Abstract:The ORF of grass carp growth hormone (cGH) was isolated and cloned from pGEX4T-1- cGH plasmid and inserted into pPIC9K, expression vector. The Pichia pastoris yeasts strain GS115 were transformed by electrophoration with pPIC9K- cGH and were screened on medium with G418. Colony PCR results showed that cGH gene had been intetrated into the yeast chromosomes. By analyses of the SDS-PAGE and Western blotting, it was showed that cGH Protein was expressed in Pichia pastoris GS115.
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