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Issue:ISSN 1000-7083
          CN 51-1193/Q
Director:Sichuan Association for Science and Technology
Sponsored by:Sichuan Society of Zoologists; Chengdu Giant Panda Breeding Research Foundation; Sichuan Association of Wildlife Conservation; Sichuan University
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Your Position :Home->Past Journals Catalog->2006 Vol.25 No.1

Analysis of DAPI Karyotype of Bighead Carp (Aristichthys nobilis)
Author of the article:KONG Qing-liang, LI Zong-yun*, FU Mei-li, WANG Qin, MANG Ying, WANG Hong-yu
Author's Workplace:(School of Life Science, Xuzhou Normal Univeisity, Xuzhou, Jiangsu Province 221116)
Key Words:Aristichthys nobilis; DAPI; heterochromatin; chromosome
Abstract:The Aristichthys nobilis chromosomes were prepared by kidney cells and stained using fluorescence dye DAPI (4’, 6’-diamidino-2-phenylindole). Then the daryotype and the distribution of chromatin of Aristichthys nobilis were studied. The results showed that the chromosome number of diploid was 48 and the karyotype formula is 30M+14SM+2ST+2T. The well-proportioned fluorescence brightness was detected in interphase nucleus, which indicated that the heterochromatin distribution in interphase nucleus was relatively symmetrical. But the bright regions lied on the short arm of chromosome 1 and 4, and they distributed at the centromere of the other chromosomes. These revealed that heterochromatin was mainly concentrated on the short arm of chromosome 1 and 4, while mainly distributed at the centromere of the other chromosomes.
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