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Issue:ISSN 1000-7083
          CN 51-1193/Q
Director:Sichuan Association for Science and Technology
Sponsored by:Sichuan Society of Zoologists; Chengdu Giant Panda Breeding Research Foundation; Sichuan Association of Wildlife Conservation; Sichuan University
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Your Position :Home->Past Journals Catalog->2006 Vol.25 No.1

Effect of Fusion Gene of Porcine Interleukin 6 and 4 on the Immunity of Mice
Author of the article:LI Hua, XIE Zhao, GAO Rong*, WU Mei, MENG Min-jie, ZHANG Huan, CHEN Chi, YANG Yi, LIU Di-guang, WANG
Author's Workplace:(Key Lab for Biological Resource and Ecological Environment of Education Ministry, College of Life Sciences, Sichuan University, Chengdu 610064)
Key Words:poreine IL6 and IL4; fusion gene; chitosan-nanoparticle (CNP); immunity; mouse
Abstract:Objective To research the immunopotentiation of a novel fusion gene of porcine interleukin-6 and -4 on immunity of mice. Methods The eukaryotic expression plasmid of fusion gene of porcine interleukin-6 and -4 was entrapped with chitosan-nanoparticle (CNP) prepared by the method of ionic cross linkage to immunize Kunming mice at the age of 21 days, and then the experimental mice were orally challenged with virulent E. coli bacteria 28 days post-inoculation. The peripheral blood were weekly collected from the tail vein of inoculated mice to detect the content of IgG, IgA, IgM and specific antibody against E.coli as well as the levels of interleukin 2 (IL2), IL4 and IL6 by the SABC-ELISA. The number of lymphocytes, monocytes, granulocytes and white blood cells were also counted out as the routine method. Results The humoral land cellular immune values were respectively elevated to different extent in the mice vaccinated with VPIL46 or VPIL4+6. The immunized mice all survived without symptoms and lesions after challenge, while the control mice appeared evident symptoms and hemorrhagic lesions mainly in digestive tissues and organs. Conclusion The PIL46 fusion gene could significantly promote the humoral and cellular immunity and resistance of mouse against E. coli infection, and would be utilized as an effective adjuvant to improve the immunity and resistance of animals against infectious diseases.
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