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Issue:ISSN 1000-7083
          CN 51-1193/Q
Director:Sichuan Association for Science and Technology
Sponsored by:Sichuan Society of Zoologists; Chengdu Giant Panda Breeding Research Foundation; Sichuan Association of Wildlife Conservation; Sichuan University
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Your Position :Home->Past Journals Catalog->2006 Vol.25 No.1

Effects of Cation on Mouse 2-cell Embryos Electrofusion
Author of the article:DI Ke-qian, LI Xiang-yun
Author's Workplace:(College of Animal Science and Technology, Agricultural University of Hebei, Baoding 071001)
Key Words:mouse; 2-cell embryo; cation; electrofusion
Abstract:The production of tetraploid embryos has become a useful tool for mouse developmental biologists within the past decade and is increasingly being used in other mammalian systems. Most notably, tetraploid embryos are commonly used to rescue embryonic lethality as a result of defective extraembryonic phenotypes in laboratory mouse strains, as well as a method of generating mice directly from embryonic stem cells. Currently, the most commonly used method of producing mouse tetraploid embryos is electrofusion by electrica stimulation. Several factors influence the efficiency of the electrofusion. In addition to the alignment of the embryos with respect to the electric field and electric field strength, the other key factor is the concentration of cation in pulsing medium. The efficiency of 6 kinds of cation on mouse 2-cell embryo electrifuston were studied in the present paper, two square DC pulses of 100V/mm and duration of 50μsec were applied when electrical stimulation. The results showed that divalent cations were necessary to achieve embryo electrofusion, and univalent cations were an inhibitor affecting the embryo electrofusion. The decreated electrofusion rate and the increased death rate of 2-cell embryos were observed when the concentration of each cation was evidently raised.
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