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Issue:ISSN 1000-7083
          CN 51-1193/Q
Director:Sichuan Association for Science and Technology
Sponsored by:Sichuan Society of Zoologists; Chengdu Giant Panda Breeding Research Foundation; Sichuan Association of Wildlife Conservation; Sichuan University
Address:College of Life Sciences, Sichuan University, No.29, Wangjiang Road, Chengdu, Sichuan Province, 610064, China
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Your Position :Home->Past Journals Catalog->2006 Vol.25 No.3

Study on Procedure of Mice Nuclear Transfer
Author of the article:WANG Feng*, JIANG Xiao-ming
Author's Workplace:(The Center of Animal Embryo Engineering and Technology of Nanjing Agricultural University, Nanjing 210095)
Key Words:mouse; nuclear transfer
Abstract:Three procedures of nuclear transfer in mice were studied. They all referred the first polar body to orient for enucleating the nuclei, so the rates of nuclei enucleation were not different. Oocytes were extraordinarily sensitive to the conventional micromanipulation, which generally resulted in cell lysis. Cutting zona pellucida beforehand could make manipulation more foftly to raise the live rate of manipulated oocytes. Pizeo-actuated micromanipulation was the best method for mice nuclear transfer, but it needed more delicate manipulation, which was not easy to master. Intracytoplasmic nuclei injection was more effective than cell electro-fusion method on reconstituting embryos of nuclear transfer.
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