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Issue:ISSN 1000-7083
          CN 51-1193/Q
Director:Sichuan Association for Science and Technology
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Your Position :Home->Past Journals Catalog->2006 Vol.25 No.3

Molecular Phylogeny of Main Groups of Wolf Spiders (Lycosidae)in China from 12S rDNA
Author of the article:LUO Yu-fa1,2, YAN Heng-mei1*
Author's Workplace:(1.Department of Zoology, College of Life Sciences, Hunan Normal University, Changsha 410081; 2. Department of Chemistry and Life Sciences, Gannan Teachers’ College)
Key Words:Araneae; Lycosidae; 12S rDNA; molecular phylogeny
Abstract:A comparison of the homologous DNA-sequences of our 6 species (belonging to 4 genera of the family Lycosidae) to 2 relative Chinese species of Lycosidae from GenBank is depicted based on analysis of the 12S rDNA in this paper. The used frequency of nucleotide was calculated and 2 molecular phylogenetic trees constructed by Mega 2.0 and PAUP microsofts respectively. The evolutionary relationships of our 6 species wolf spiders were investigated by comparing the using two different analysed methods (Neighbor-Joining, NJ; and Maximum Parsimony, MP) and two different outgroups. In the mean 291.6bp fragments of mitochondrial 12S rDNA, A+T was about 78.13% and G+C only21.87%. The sequence data revealed considerable variation in 161 nucleotide sites among the analyzed individuals from 5 different genera. The most variation rate of transversion was larger than or approached to that of transition. The results showed that the ranges of the 12S rDNA nucleotide divergence within species, among species of a genus and among genera of a family were 1.08% and 6.85%14.80% respectively. The members of the same genus and family were grouped together. The phylogenetic relationship of 3 subfamilies Lycosinae was: Hippasinae→Lycosinae→Pardosinae.The phylogenetic relationship of 5 genera of Lycosidae was: PirataHippasa (or Pirata and Hippasa) →TrochosaLycosaPardosa. Pirata was divided firstly, or Pirata and Hippasa were grouped together firstly as a subfamily (Hippasinae),which differs from the recent taxonomy distinctly. The 4 examined species of Pardosa was grouped together between two methods of phylogenetic analysis (NJ and MP), which is the most evolutive group, as same as recent taxonomy. Lycosa was more evolutive than did Trochosa, as reverse as recent taxonomy.
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