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Issue:ISSN 1000-7083
          CN 51-1193/Q
Director:Sichuan Association for Science and Technology
Sponsored by:Sichuan Society of Zoologists; Chengdu Giant Panda Breeding Research Foundation; Sichuan Association of Wildlife Conservation; Sichuan University
Address:College of Life Sciences, Sichuan University, No.29, Wangjiang Road, Chengdu, Sichuan Province, 610064, China
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Your Position :Home->Past Journals Catalog->2011 Vol.30 No.2

A Study on -80℃ Cryopreservation Techniques of Monopterus albuus Spermatozoa
Author of the article:YAN Xiu-ming1, ZHANG Lin-da2, ZHANG Xiao-xue1*
Author's Workplace:(1.Guizhou Key Laboratory of Animal Genetics Breeding and Reproduction, College of Animal Science, Guizhou University, Guiyang 550025, China; 2. Bijie Institute of Animal Husbandry and Veterinary, Bijie, Guizhou Province 551700, China)
Key Words:Monopterus albuus; sperm; cryopreservation
Abstract:In this paper, the -80℃ cryopreservation techniques of Monopterus albuus spermatozoa were studied. The following results were obtained: Before -80℃ cryopreservation, a lower-temperature equilibrium process was not needed; if 10% DMSO was used as an anti-freezing protective agent, the sperm relative activity was higher than that of others; if 10% DMSO was used as an anti-freezing protective agent and 200 μL centrifuge tubes used as freezing containers, the sperm relative activity increased to 79% when preserved 168 h; Moreover, when thin tubes were used as containers, the sperm relative activity was 88%, when preserved the same period of time. The results of the study would be an experimental basis for the establishment of a Monopterus albuus sperm cryopreservation library.
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