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Issue:ISSN 1000-7083
          CN 51-1193/Q
Director:Sichuan Association for Science and Technology
Sponsored by:Sichuan Society of Zoologists; Chengdu Giant Panda Breeding Research Foundation; Sichuan Association of Wildlife Conservation; Sichuan University
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Your Position :Home->Past Journals Catalog->2011 Vol.30 No.2

Molecular Sex Identi?cation of Grus nigricllis
Author of the article:YU Hai-long, ZHANG Jian-feng, HE Zong-liang, JIA Xiao-xu, DU Wen-xing*
Author's Workplace: (College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China)
Key Words:Grus nigricllis; sex determination; non-invasive sampling
Abstract:Grus nigricllis is not a kind of sexually dimorphic bird, it’s difficult to identify the sex morphologically. In this paper, genomic DNA was extracted from feathers using a non-invasivesampling method. A PCR test was used employing P2-P8 primers that amplify the CHD gene specifically. The PCR productsof male and female appear as almost the same size band in electrophoresis analysis. Additionally, the PCR products were digested with HaeIII and Asp700I for RFLP analysis. Male PCR products showed two bands after digestion with HaeIII, while females showed three bands. Male PCR products showed a unique band after digestion with Asp700I, while females showed three bands. Results from these two tests can accurately identify the sex of G. nigricllis. In this paper, a method of accurately sexing G. nigricllis was established, while extraction of DNA from feathers using a non-invasivesampling method, a PCR test and RFLP analysis were used. This method laid a foundation for rational allocation of female-male proportions and improving reproduction ratios when artificial propagation of G. nigricllis is adopted.
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