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Issue:ISSN 1000-7083
          CN 51-1193/Q
Director:Sichuan Association for Science and Technology
Sponsored by:Sichuan Society of Zoologists; Chengdu Giant Panda Breeding Research Foundation; Sichuan Association of Wildlife Conservation; Sichuan University
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Your Position :Home->Past Journals Catalog->2010 Vol.29 No.6

Immunohistochemical Localization of 5-HT Cells in Gastrointestianl Tract of Phasianus colchicas
Author of the article:LI Li-Xia
Author's Workplace:(School of Biological Sciences & Engineering, Shanxi University of Technology, Hanzhong, Shanxi Provence 723000, China)
Key Words:Phasianus colchicas;Gastrointestinal tract;5-HT cells;Immunohistochemistry
Abstract:Objective  to investigate the distributed patterns(density) and morphologies of 5-hydroxytryptamine (5-HT) immunoreactive cells in the gastrointestinal tract of Phasianus colchicas by using anti-5-HT serum and the origin of their distribution pattern as well as the relationship between cell morphology and function had been discussed.  Mathods  The method of the ABC(avidin-biodin- peroxidase complex) immunhistochemical technique was used.  Results 5-HT cells were mainly found in the duodenum and fundus, rarely in the glandular stomach, the density curve appeared undulation. the density of 5-HT cells was the highest in the jejunum and duodenum, secondly in the caecum and ileum, thirdly in the rectum. The 5-HT cells had different shapes including round, ellipse,shuttle-shaped, the 5-HT cells mainly distributed in the lamina propria, between epithelial cells, at the bottom of epithelial cells, or between glandular epithelial cells. Conclusion The evidences collected in this paper indicate the origin of distributive mode of 5-HT cells in the gastrointestinal tract of Phasianus colchicas may be related to different digestive function of different region. According to the morphology of 5-HT cells, the writer suggested that 5-HT cells in the gastrointestinal tract of Phasianus colchicas had both endocrine and exocrine functions. 
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