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Issue:ISSN 1000-7083
          CN 51-1193/Q
Director:Sichuan Association for Science and Technology
Sponsored by:Sichuan Society of Zoologists; Chengdu Giant Panda Breeding Research Foundation; Sichuan Association of Wildlife Conservation; Sichuan University
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Your Position :Home->Past Journals Catalog->2010 Vol.29 No.4

Application of Species-specific PCR Primers and DNA Sequencing in the Forensic Identification of Two Monitors
Author of the article:WANG Li-li, PENG Jian-jun*, YU Dong-mei, HU Shi-jia, XIN Cui-na, ZHANG Yu-shu
Author's Workplace:(South China Institute of Endangered Animals, Guangzhou 510260, China)
Key Words:species identification; monitor lizard; COI gene; species-specific primers; DNA sequencing
Abstract:In the paper, two species of monitors, Varanus salvator and Varanus bengalensis, were identified using two methodologies of species-specific PCR primers and DNA sequencing. Partial fragments of the COI gene were amplified from two monitor lizards using universal primers and species-specific primers. The results showed that species-specific primers and universal primers could be applied in distinguishing the two monitors. In addition, the applicable condition of the two identification methodologies were discussed. These methodologies satisfy the needs of species identification for police cracking down on smuggling.
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