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Issue:ISSN 1000-7083
          CN 51-1193/Q
Director:Sichuan Association for Science and Technology
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Your Position :Home->Past Journals Catalog->2009 Vol.28 No.5

Osteogenic Differentiation of Mouse MC3T3-E1 Cells on Self-assembling Peptide Hydrogel Scaffolds
Author of the article:LIN Juan, ZHOU Qing-han, ZHAO Xiao-jun*
Author's Workplace:(Institute for Nanobimedical Technology and Membrane, Sichuan University, Chengdu 610041, China)
Key Words: MC3T3-E1 cells; osteogenic differentiation; self-assembling peptide hydrogels; tissue engineering
Abstract:
Objective Mouse MC3T3-E1 cells growing and differentiating in a self-assembling peptide hydrogel were investigated in order to testify that peptide hydrogel was characterized as the top scaffold for bone tissue engineering. Methods MC3T3-E1 were seeded into RADA16 hydrogel, the viability was visualized and detected under fluorescence microscopy. Further cultured in osteogenic medium for 2~3 weeks, alkaline phosphatase activity and calcium deposits were evaluated by histochemical techniques. RT-PCR analyses were preformed to detect the expression of mRNA transcripts. Results The percentage of living cells in RADA16 hydrogels was over 80%. The cells had a typical spindle shape. High alkaline phosphatase activity and calcium deposits were detected after osteogenic induction. The expression of OPN and BSP at the mRNA level was also detected. Conclusion MC3T3-E1 can differentiate into osteoblasts to form mineralized matrices within the hydrogel scaffold.
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