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Issue:ISSN 1000-7083
          CN 51-1193/Q
Director:Sichuan Association for Science and Technology
Sponsored by:Sichuan Society of Zoologists; Chengdu Giant Panda Breeding Research Foundation; Sichuan Association of Wildlife Conservation; Sichuan University
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Your Position :Home->Past Journals Catalog->2009 Vol.28 No.5

Isolation, Culture and Identification of Rat Bone Marrow-Derived Endothelial Progenitor Cells
Author of the article:ZHAO Ying, TANG An-ke
Author's Workplace: (Chongqing Normal University, The Key Laboratory of Animal Biology of Chongqing, Chongqing, 400047, China)
Key Words:rat; endothelial progenitor cells; isolation; culture; identification
Abstract:
Objective To study the methods of isolation, culture and identification of endothelial progenitor cells (EPCs) from rat bone marrow. Methods Mononuclear cells suspension of rat bone marrow was prepared by density gradient centrifugation on Percoll (1.077 g/ml). The mononuclear cells were induced by VEGF and bFGF, and then the shape of these cells were observed by light microscopy. EPCs were identified by immunofluorescence staining for the expression of PECAM-1/CD31, VE-cadherin/CD144, fluorescein Ulex Europaeus agglutinin-1 (FITC-UEA-1) and uptake of DiI complexed acetylated low-density lipoprotein (Dil-ac-LDL). Results The clone-like morphology and the “cobblestone” structure were observed by inverted microscopy after 7 days cultivation. Laser Scanning Confocal Microscopy (LSCM) showed that the adherent cells were positive for CD31 and VE-cadherin. Moreover the cells could incorporate Dil-ac-LDL and bind FITC-UEA-1. Conclusion 1.077 g/ml percoll density centrifugation, with VEGF and bFGF induction may obtain EPCs from rat bone marrow, thus demonstrating the feasibility of the methods used.
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