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Issue:ISSN 1000-7083
          CN 51-1193/Q
Director:Sichuan Association for Science and Technology
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Your Position :Home->Past Journals Catalog->2009 Vol.28 No.5

Enhanced Immunity of Pigs to Foot and Mouth Disease Vaccine by Inoculation with Shuffled Pig IL-2 Gene and Recombinant IL-6C Plasmids
Author of the article:ZHENG Min*, CHEN Wen-long1*, YANG Xiao1*, CHENG Chi2, LI Dong1, WANG Ze-zhou1, ZHANG Hua-bing1, LI Y
Author's Workplace:(1.Key Laboratory for Bio-Resource and Eco-Environment of Ministry Education, College of Life Sciences, Sichuan University, Chengdu 610064, China; 2. Bioengineering Department, Sichuan University of Science and Engineering; 3. Sichuan Provincial Academy of Animal Science; 4. Clinical Laboratory of Sichuan University Hospital)
Key Words: swine; IL-2 gene; IL-6 gene; CpG; immunity; vaccine of foot and mouth disease
Abstract:
The recombinant shuffled pig IL-2 gene and IL-6 gene plasmids with CpG motifs inserted were entrapped with chitosan nanoparticles (CNP) prepared by the ionic cross linkage method (CNP-VRIL2S-VRIL6C), and were then injected intramuscularly to inoculate hybrid swine which were co-immunized with the inactivated foot and mouth disease vaccine (FMDV). Blood was collected from the pigs on 7, 14, 28, 42, and 56 days post vaccination to detect immunoglobulin changes, specific antibodies, IL-2, IL-4, IL-6, and immune cells. The results showed that from 7 to 56 days after vaccination, the specific antibody, IgG, IgA and IgM significantly increased in the treated pigs in comparison with those of the control swine(P<0.05); the amounts of IL-2, IL-6, monocytes and lymphocytes were remarkably elevated in the blood of the treated group as compared with those of the control pigs. This suggests that CNP-VRIL2S-VRIL6C boosts the specific humoral and cellular immune responses of pigs to FMDV, implying that CNP-VRIL2S-VRIL6C could promote the resistance of the swine against FMD and is a putatively promising effective immunoadjuvant to facilitate the control of FMD in pigs.
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