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Issue:ISSN 1000-7083
          CN 51-1193/Q
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Your Position :Home->Past Journals Catalog->2009 Vol.28 No.4

Establishment of a Mouse Pneumonia Model for Mycoplasma pneumoniae Infection in Different Time and Doses
Author of the article:YANG Chun1,3 ,ZHOU Li2, HE Yong-lin1,3
Author's Workplace:(Department of Microbiology1, Institute of Neuroscience 3Chongqing Medical Univercity,Chongqing 400016, Tianjin Infectious Disease Hospital2, Tianjin 300192 )
Key Words:Mycoplasma pneumoniae; pathological changes; time and dosis; animal model
Abstract:
Objective To establish a mouse pneumonia model forMycoplasma pneumoniae (MP) infection at different times and doses, and examine the progression of the pneumonia in mice. Methods BALB/c mice were randomly grouped. Group was inoculated intranasally with MP suspension on days 0, 1, and 2. Group was inoculated on days 0, 8, and 9. Control groups were set up with sterile normal saline. Mice were sacrificed on 3~18 days. MP was detected by culturing the lung tissues and brochoalveolar lavage fluid (BALF) of each mouse. Lung tissue sections were examined and a histopathological scoring system was applied to judge the severity of the inflammation. Results All of the mice survived after inoculation. The infected animals showed inflammatory changes in the lungs varying grades. The MP cultures of lung and BALF specimens from infected animals were positive and negative from the control groups. Histopathologic scores ranged from 1.5 to 14.3. Conclusion The mouse pneumonia model for MP infection was established successfully and the histopathological scoring system can be used to evaluate the severity of acute inflammatory lesions of the lung on BALB/c mice. The model provided an experimental basis for further study of the pathogenesis and immunological state after MP infection.
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