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Issue:ISSN 1000-7083
          CN 51-1193/Q
Director:Sichuan Association for Science and Technology
Sponsored by:Sichuan Society of Zoologists; Chengdu Giant Panda Breeding Research Foundation; Sichuan Association of Wildlife Conservation; Sichuan University
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Your Position :Home->Past Journals Catalog->2022 Vol.41 No.3

Mitochondrial Genome Characterization and Phylogeny of Diplodiscus nigromaculati
Author of the article:LI Zongxian1, LI Jiqian1, XU Weijiang1,2, FAN Lixian1,2*
Author's Workplace:1. School of Life Science, Yunnan Normal University, Kunming 650500, China;
2. Engineering Research Center of Sustainable Development and Utilization of Biomass Energy, Ministry of Education, Kunming 650500, China
Key Words:Diplodiscus nigromaculati; Paramphistomoidea; Diplodiscidae; mitochondrial genome; new host record
Abstract:Trematodes of family Diplodiscidae Cohn, 1904 are common parasites of amphibian, whose morphological diversities have widely been reported. However, the research on the genetic diversities based on mitochondrial genome is still undocumented. The complete mitochondrial genome of Diplodiscus nigromaculati (Diplodiscus Diesing, 1836) from the intestinal tract of host Dianrana pleuraden was described and analyzed in the present study. The complete mitochondrial genome of D. nigromaculati is 14697bp long (GenBank accession number:MW698822), which is composed of 22 tRNA genes, 12 protein-coding genes, 2 rRNA genes and 1 noncoding region. The content of AT (60%) in the mitochondrial genome is higher than that of GC (40%), which is the lowest in reported species of Paramphistomoidea Fischoeder, 1901. The exchange of trnG and trnE indicate an important genetic structure feature of mitochondrial genome of flukes of the Paramphistomoidea. The phylogeny analysis using concatenated amino acid sequences for 12 protein-coding genes supported that Diplodiscus belongs to Diplodiscidae. This study indicated that D. pleuraden is the new host record of D. nigromaculati. These results provided basic data and reference for the future research of genetic structure and genetic diversity of this group of fluke.
2022,41(3): 248-255 收稿日期:2021-11-01
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