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Issue:ISSN 1000-7083
          CN 51-1193/Q
Director:Sichuan Association for Science and Technology
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Your Position :Home->Past Journals Catalog->2022 Vol.41 No.3

Effect of Ficus hispida Fruit on Fecal Microorganisms of Centrochelys sulcata
Author of the article:ZHANG Xiaoying1#, ZOU Jiejian2#, XIAO Jiajie2, LU Yuyan1*, LI Pipeng1*, GU Hexiang2, YANG Guangda2, HOU Fanghui2, WANG Xueqian3
Author's Workplace:1. Institute of Herpetology, Shenyang Normal University, Shenyang 110034, China;
2. Guangdong Wildlife Monitoring and Rescue Center, Guangzhou 510000, China;
3. Shenyang Children's Hospital, Shenyang 110033, China
Key Words:Centrochelys sulcata; Ficus hispida; faeces; microorganisms; function prediction
Abstract:To better understand the effect of food on the intestinal flora of Centrochelys sulcata under captive conditions. In this study, we used V3-V4 region of 16S rRNA gene amplicon sequencing to analyze the changes of fecal microflora and its functional predictions in 7 similar-sized C. sulcata before and after autonomously feeding on Ficus hispida fruit. The results showed that the Alpha diversity indexes and dominant phyla and their relative abundance did not differ significantly between groups. The number and relative abundance of genera with relative abundance >1% differed significantly. Principal co-ordinates analysis and hierarchical clustering analysis revealed significant separation of groups. LEfSe analysis showed that both groups had their own marker species. The results of KEGG metabolic pathway analysis showed that, the fecal flora of fruit eating group enriched significantly higher amount of genes synthesizing antibiotics such as tetracyclines, polyketides and the antioxidant active substance betalain, and the genes degrading environmental pollutants such as ethylbenzene, bisphenol, atrazine and polycyclic aromatic hydrocarbons. This study suggests that the C. sulcata intestinal flora has the ability to positively respond to food change.
2022,41(3): 241-247 收稿日期:2021-11-30
分类号:S864
基金项目:国家林业局项目(2130211)
作者简介:张笑颖(1997—),硕士研究生,研究方向:动物结构与功能的演化,E-mail:2857382953@qq.com;邹洁建(1971—),高级工程师,研究方向:野生动物保护,E-mail:zjj.020207@163.com
*通信作者:陆宇燕,E-mail:185570795@qq.com;李丕鹏,E-mail:104466606@qq.com
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