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Issue:ISSN 1000-7083
          CN 51-1193/Q
Director:Sichuan Association for Science and Technology
Sponsored by:Sichuan Society of Zoologists; Chengdu Giant Panda Breeding Research Foundation; Sichuan Association of Wildlife Conservation; Sichuan University
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Your Position :Home->Past Journals Catalog->2022 Vol.41 No.1

Transcriptome Assembly and Annotation of Arborophila rufipectus
Author of the article:LIU Yi1, HE Lewei1, LAN Yue1, ZHOU Chuang1, CHEN Benping2, YUE Bisong1,3, MENG Yang1,3*
Author's Workplace:1. Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu 610065, China;
2. Laojunshan National Nature Reserve Administration, Pingshan, Sichuan Province 645350, China;
3. Sichuan Key Laboratory of Conservation Biology on Endangered Wildlife, College of Life Sciences, Sichuan University, Chengdu 610065, China
Key Words:Arborophila rufipectus; transcriptome; assembly; annotation
Abstract:Sichuan hill-partridge (Arborophila rufipectus) is a rare and endangered bird endemic to China. In this study, transcriptome sequencing, assembly and annotation was performed on the heart, liver and kidney of one male adult individual. The heart, liver and kidney produced 5.70 G, 4.60 G and 5.16 G data respectively after filtration. In total, 234 488 genes were assembled by Trinity after redundancy removed from 286 661 transcripts. BUSCO assessed the assembly integrity as 97.6%. 81.29% of unigenes contained no open reading frame(ORF), 16.43% contained 1 ORF, and 2.28% contained 2 or more ORFs. 70 737 genes were annotated in the NR, GO, KEGG, Swiss and COG databases, accounting for 30.17%, of which the NR database accounted for the highest proportion (29.27%), and the COG database had the least annotation results (4.37%). There are 6 998 genes annotated in all 5 databases, and the NR database had the most individually annotated genes (23 773). This research provides basic data for exploring of the functional genes of A. rufipectus, and provides genetic data for the conservation of this species.
2022,41(1): 24-29 收稿日期:2021-06-04
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