Abstract：Morphology and DNA barcode of
Nisia fuliginosa, Yang & Hu, 1985, a new insect pest of
Schoenoplectus tabernaemontani in the Caohai Wetland National Nature Reserve, Weining, Guizhou Province, were reported. The morphological characteristics of
N. fuliginosa (eggs, nymphs and adults) were described in detail. The
COⅠ sequence of
N. fuliginosa were obtained by PCR, sequence compositions and genetic distances were analyzed, and the phylogenetic tree of 3 meenoplid species constructed by MEGA 6.0 using Neighbor-Joining methods was provided,
Nisia sp. and
Phaconeura sp. 2 species were chosen as outgroups. The results showed that the
COⅠ sequences of 6
N. fuliginosa samples were clustered in the same evolutionary branches, and clearly distinguished from other species. The intraspecific genetic distances of
N. fuliginosa was 0, whereas the interspecific genetic distances of the 3 meenoplid species was 0.166 8-0.236 1 and no overlapped genetic distance was detected between intraspecific and interspecific. Moreover, the damage symptoms of
N. fuliginosa were introduced. This study suggests that the sequence of
COⅠ gene can be used as a DNA barcode for the fast and accurate identification of the eggs, nymphs and adults of
N. fuliginosa.
2021,40(2): 130-141 收稿日期：2020-07-04
分类号：Q969.35;S433.3
基金项目：贵州省高层次创新型人才项目（黔科合人才20154021）
作者简介：吕莎莎(1998-),硕士研究生,研究方向:节肢动物系统学与分子生物学,E-mail:ls13116322648@163.com
*通信作者：陈祥盛,E-mail:chenxs3218@163.com
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