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Issue:ISSN 1000-7083
          CN 51-1193/Q
Director:Sichuan Association for Science and Technology
Sponsored by:Sichuan Society of Zoologists; Chengdu Giant Panda Breeding Research Foundation; Sichuan Association of Wildlife Conservation; Sichuan University
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Your Position :Home->Past Journals Catalog->2020 Vol.39 No.6

Differential Transcriptome Analysis in Multi-Tissue of Bufo gargarizans
Author of the article:XU Wencai1, LIN Zhuyang1, XIAO Yadan1, TANG Ruixiang1, FAN Zhenxin1,2, MENG Yang1,2*
Author's Workplace:1. Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu 610065, China;
2. Sichuan Key Laboratory of Conservation Biology on Endangered Wildlife, College of Life Sciences, Sichuan University, Chengdu 610065, China
Key Words:Bufo gargarizans; transcriptome; retroauricular gland; RNA-Seq
Abstract:Venenum bufonis, the skin products of Chinese toad (Bufo gargarizans) and toad-cortex, its sloughed skin has an extremely high medicinal value. Modern medical research has identified and proved that they contain a large variety of effective biological components. In our research, RNA-Seq is sequenced to analyze the transcriptome of a toad's 7 tissues in total, including liver, spleen, heart, follicles, muscles, abdominal skin and retroauricular gland of the right ear. Next the de novo method is used to complete the assembly and construction of multi-tissue transcripts of B. gargarizans, thus a highly representative transcriptome is obtained. Then by performing homology comparisons among major protein databases, the transcriptome functional annotation of the multi-tissues in B. gargarizans is completed. By calculating and comparing the expression levels of transcripts in various tissues, a total of 3 668 significantly up-regulated genes and 260 significantly down-regulated genes are obtained between the retroauricular gland and other tissues of B. gargarizans. The functional enrichment analysis of the above-mentioned differentially expressed genes find that they are significantly enriched in protein synthesis, enzyme regulation, molecular transmembrane transporter generation, and metabolic synthesis pathways of precursor substances, suggesting that there is a significant advantage in the synthesis and metabolism of corresponding steroids in the retroauricular gland of B. gargarizans. These data in this study provide a multi-tissue transcriptome of B. gargarizans, and provide support for more in-depth studies on the gene expression of B. gargarizans in the future. They also provide a valuable resource for further research on the toad secretions of B. gargarizans, which can help to improve the research on the medicinal value of venenum bufonis and toad-cortex.
2020,39(6): 601-615 收稿日期:2020-07-22
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