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Issue:ISSN 1000-7083
          CN 51-1193/Q
Director:Sichuan Association for Science and Technology
Sponsored by:Sichuan Society of Zoologists; Chengdu Giant Panda Breeding Research Foundation; Sichuan Association of Wildlife Conservation; Sichuan University
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Your Position :Home->Past Journals Catalog->2008 Vol.27 No.6

Construction and Expression in vitro of Prokaryotic Expressional Vector of Trichomonas vaginalis Adhesion Protein 33 Gene
Author of the article:YANG Shu-guo1, WANG Ya-jing2*, ZHU Xiao-yan2, TIE Chao-nan2, LIAO Lin2
Author's Workplace:(1. Department of Parasitology, Yunyang Medical College, Shiyan, Hubei Province 442000, China; 2. Department of Parasitology, School of Preclinical and Forensic Medicine, Sichuan University)
Key Words:Trichomonas vaginalis; adhesion protein 33; prokaryotic expressional vector; gene expression
Abstract:Objective  To construct the prokaryotic expressional vector of adhesion protein 33(ap33) on the isolate of Trichomonas vaginalis and induce the expression of ap33 gene in vitro. Methods  The plasmid pMD-18T-ap33 and pUC18 were digested by BamH Ⅰ and XbaⅠ. The ap33 gene was subcloned into the plasmid pUC18 and induced to express AP33 in prokaryotic cell by IPTG, which was analyzed by SDS-PAGE and Western-blot. Results  The correct recombinant plasmid pUC18-ap33 was isolated and confirmed by PCR and restriction analysis. The relative molecular mass of AP33 was almost Mr 36 000. Conclusion  The recombinant plasmid pUC18-ap33 was constructed successfully and the adhesion protein 33 could be induced to express in vitro.
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