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Issue:ISSN 1000-7083
          CN 51-1193/Q
Director:Sichuan Association for Science and Technology
Sponsored by:Sichuan Society of Zoologists; Chengdu Giant Panda Breeding Research Foundation; Sichuan Association of Wildlife Conservation; Sichuan University
Address:College of Life Sciences, Sichuan University, No.29, Wangjiang Road, Chengdu, Sichuan Province, 610064, China
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Your Position :Home->Past Journals Catalog->2008 Vol.27 No.6

Potentializtion of Immunity to PRRSV by Inoculation with Fusion Gene of Pig IL-4, IL-6 and CpG Motifs
Author of the article:CHEN Jian-lin1*, YANG Xiao1*, ZHANG Huan2*, LI Dong1, CHENG Chi3, ZHAO Zhong-zhong1, CHEN Qian1, ZHA
Author's Workplace:(1. Key Laboratory for Bio-Resource and Eco-Environment of Ministry Education, College of Life Sciences, Sichuan University, Chengdu 610064, China; 2. Biology Group of North Sichuan Medical College; 3. Bioengineering Department of Sichuan University of Science & Engineering)
Key Words:pig; porcine IL-6 and IL-4 fusion gene; CpG motifs; PRRS; vaccination
Abstract: In order to develop safe and effective immunoadjuvant for porcine reproductive and respiratory syndrome vaccine (PRRSV), the recombinant eukaryotic expression plasmid for the fusion gene of porcine IL-6 and IL-4 (PIL-46), and specific CpG Oligodeoxynucleotides (ODN) were packed with chitosan nanoparticles(CNP) prepared by ionic cross linkage, named as CNP-(VRIL-46+VR1C). Then the CNP-(VRIL-46+VR1C) was intramuscularly inoculated into Landrace and Chuanbai hybrid pig at the age of 14 days which were simultaneously immunized with inactivated porcine reproductive and respiratory syndrome vaccine. The peripheral blood of experimental pigs were collected at 1, 2, 4, 6 and 8 weeks post inoculation to detect the content of immunoglobulins, specific antibody and IL-2, IL-4 and IL-6 by the Sandwich ELISA. The results showed that from the 1 week to 8 weeks post vaccination, the levels of immunoglobulins, cytokines, the specific antibodies as well as the numbers of lymphocytes significantly increased in the blood of vaccinated pigs inoculated with CNP-(VRIL-46+VR1C) in comparison with those of control group. These findings suggest that CNP-(VRIL-46+VR1C) could significantly enhance the specific humoral and cellular immunity of porcine to PRRSV, indicating that the CNP-(VRIL-46+VR1C) could be utilized as a novel effective adjuvant to improve the immunity and resistance of animals against PRRSV.
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