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Issue:ISSN 1000-7083
          CN 51-1193/Q
Director:Sichuan Association for Science and Technology
Sponsored by:Sichuan Society of Zoologists; Chengdu Giant Panda Breeding Research Foundation; Sichuan Association of Wildlife Conservation; Sichuan University
Address:College of Life Sciences, Sichuan University, No.29, Wangjiang Road, Chengdu, Sichuan Province, 610064, China
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Your Position :Home->Past Journals Catalog->2008 Vol.27 No.6

Phylogenetic Relationships of Common Calyptratae Flies (Insecta: Diptera) Based on Sequences of 28S rRNA Gene
Author of the article:GE Zhen-ping,ZHI Yan,ZHANG Chun-tian*
Author's Workplace:(Shenyang Normal University, Laboratory of Biodiversity and Evolution, Shenyang 110034, China)
Key Words:Diptera;fly; phylogeny; 28S rDNA
Abstract:The sequences for partial 28S rRNA gene were determined for 15 species from 6 Calyptrate families in China, and the homologous of partial 28S rRNA sequences for 6 species of 5 Calyptrate families from the GenBank, their combination results in a tree representing 7 Calyptrate families. With the help of Mega3.0 software, the molecular phylogenetic trees under maximum parsimony (MP) and neighbour-joining (NJ) methods were reconstructed using Drosophilia melanogaster as outgroup. The results showed that there were 126 variable sites and 101 parsimony-informative sites in the nucleotides of the data, and the average A+T content of 28S rRNA gene in Calyptrae was 68.8% which showed a strong A+T bias. Molecular phylogenetic trees indicated that the monophyly of Calyptratae was well supported, Calyptrae were divided into two main clades in this study, Sarcophagidae were related to Calliphoridae rather than to Tachinidae. They were composed of a higher superfamily Oestroidea; Muscidae and Anthomyiidae were grouped into a group of superfamily Muscoidea. All above results matched the classical morphological taxonomy reasonably well. But Scathophagidae and Fanniidae displayed a tendency to cluster within Oestroidea. This result was significant incongruence with classical morphological taxonomy.
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