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Issue:ISSN 1000-7083
          CN 51-1193/Q
Director:Sichuan Association for Science and Technology
Sponsored by:Sichuan Society of Zoologists; Chengdu Giant Panda Breeding Research Foundation; Sichuan Association of Wildlife Conservation; Sichuan University
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Your Position :Home->Past Journals Catalog->2008 Vol.27 No.6

Influence of Mouse Genetic Background on Developmental Potential of Tetraploid Embryo
Author of the article:WEN Xiao-hui1,2, GAO Shu-min1,2, DI Ke-qian1,2, YANG Rong-rong1,2, ZHANG Peng1,2, ZHAO Hu1,2,LI Xian
Author's Workplace: ( 1. College of Animal Science and Technology, Agriculture University of Hebei, Baoding, Hebei Province 071001, China; 2. Embryo Engineering and Technological Center of Cattle and Sheep of Hebei)
Key Words:genetic background; mouse tetraploid embryos; developmental potential; electrofusion
Abstract: Developmental potential and electrofusion rate are different among mice genetic backgrounds after electrofusimg diploid blastomeres at 2-cells stage. In this study, mice diploid blastomeres at 2-cells stage with different genetic backgrounds (C57, ICR, BALB/c, B6D2F2, B6C3D2F2) were electrofused in the same condition. The results showed: electrofusion rate and developmental potential of tetraploid embryos with hybrid genetic background were higher than that of with inbred and outbred genetic (P<0.05). Tetroploid blastocysts with high heterozygosity contained much more cells compared to those with low heterozygosity (P<0.05 or P<0.01). The post-implantation rates of tetraploid blastocysts with different genetic background were not significant (P>0.05), but those with high heterozygosity can survive longer time. 5 fetus from mice tetraploid embryos with hybrid genetic background which developed to 13.5 dpc(days post coitum, dpc) were obtained, while no fetus could develop to 11.0 dpc from those with homozygous genetic background.
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