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Issue:ISSN 1000-7083
          CN 51-1193/Q
Director:Sichuan Association for Science and Technology
Sponsored by:Sichuan Society of Zoologists; Chengdu Giant Panda Breeding Research Foundation; Sichuan Association of Wildlife Conservation; Sichuan University
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Your Position :Home->Past Journals Catalog->2020 Vol.39 No.1

Development of Polymorphic Microsatellite Markers for Ailuropoda melanoleuca Based on RNA-Sequencing
Author of the article:TU Hongmei, ZHOU Chuang, WANG Guannan, CHENG Meiling, YUE Bisong, MENG Yang*
Author's Workplace:Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu 610065, China
Key Words:Ailuropoda melanoleuca; genome; transcriptome; polymorphic; microsatellite
Abstract:Based on the published genomes of giant pandas (Ailuropoda melanoleuca), transcriptome of 6 giant pandas were sequenced in this study to screen the polymorphic microsatellite loci and analyze their composition and characteristics. The results showed that a total of 326 polymorphic microsatellite loci were obtained, of which the dinucleotide polymorphism microsatellites accounted for a maximum of 69.93% (228 in total). The proportions of tri-, tetra-, penta-, and hexanucleotide microsatellite loci were 9.51%, 14.11%, 5.21%, and 1.22%, respectively. According to the 2 indicators, deletion rate and standard deviation, and the length of the sequence, 20 dinucleotide microsatellite loci were selected and verified in 25 giant panda individuals. Further analyses showed that the number of alleles at different loci ranged from 2 to 8, with an average value of 3.70. The observed heterozygosity and the expected heterozygosity ranged from 0 to 1.000 and 0.280 to 0.784 with the average values 0.472 and 0.532, respectively. After Bonferroni correction, it was confirmed that the 4 loci significantly deviated from the Hardy-Weinberg Equilibrium, and no significant linkage disequilibrium was observed at all loci (P>0.01). The polymorphic information content (PIC) of 20 loci ranged from 0.246 to 0.734, with 9 highly polymorphic loci (PIC>0.50), and 11 loci were moderately polymorphic (0.25 < PIC < 0.50). The microsatellite loci screened in this study can help to assess the genetic diversity and population structure of giant pandas, to develop effective conservation and management strategies, and provide resources for the subsequent development of more excellent microsatellite loci for the genetic study of giant panda population.
2020,39(1): 15-22 收稿日期:2019-08-07
分类号:Q959.8;Q347
基金项目:国家科技攻关项目(2016YFC0503200);国家自然科学基金项目(31702032)
作者简介:涂洪梅(1995-),女,硕士研究生,研究方向:分子生态学,E-mail:295047526@qq.com
*通信作者:孟杨,E-mail:mengyang@scu.edu.cn
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