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Your Position :Home->Past Journals Catalog->2020 Vol.39 No.1

Evolution and Expression of Liver Cell Cycle-Related Genes in Myospalax baileyi
Author of the article:AN Zhifang1,2, WEI Linna3, WANG Zhijie1,2, LI Suhua1,2, XU Bo1,4, LI Yongxiao1,4, WEI Lian4, WEI Dengbang1,2,4*
Author's Workplace:1. State Key Laboratory of Plateau Ecology and Agriculture, Qinghai University, Xining 810016, China;
2. Medical College, Qinghai University, Xining 810016, China;
3. Academy of Animal Science and Veterinary Medicine, Qinghai University, Xining 810016, China;
4. College of Eco-Environmental Engineering, Qinghai University, Xining 810016, China
Key Words:Myospalax baileyi; cell cycle-related gene; evolution analysis; altitude; expression
Abstract:The plateau zokor (Myospalax baileyi) is a specialized subterranean rodent living on the Qinghai-Tibet Plateau which has strong ability in adaptation to hypoxic environments. Hypoxia induces arrest of cell cycles G1 and G2. In order to understand the molecular mechanism of the adaptation of M. baileyi to hypoxic environments, the homologies, positive selection sites and the convergent evolution sites of p21, CyclinD1, CDK6, CyclinE, CDK2, 14-3-3-σ, Gadd45α, B99 and CyclinB1 genes were analyzed by MEGA 7.0, PAML 4.8 program and Ancestor program, respectively. In addition, the expression levels of these genes in the liver tissues under different altitudes (3 300 m and 2 260 m) were determined by real-time PCR and compared with those of SD rat (Rattus norvegicus). The results showed that, (1) the nucleotide and amino acid sequences of the cell cycle-related genes in M. baileyi had high homologies (>90%) with Nannospalax galili; p21, CyclinD1, CyclinE and CyclinB1 of M. baileyi and N. galili occurred convergent sites. SIFT test showed that 27 and 105 variation sites might influence the regulation of p21 and CyclinB1, respectively. (2) Compared to low altitude of 2 260 m, the expression level of p21 (cell cycle G1-related gene) in M. baileyi liver tissues under high altitude of 3 300 m was significant increased, while its downstream genes CyclinD1, CyclinE, CDK6 and CDK2 were significant decreased. By contrast, the expression levels of these genes were not significantly different in R. norvegicus. Moreover, no significant differences of the expression levels of cell cycle G2-related genes such as Gadd45α, B99, 14-3-3-δ and CyclinB1 were detected in M. baileyi and R. norvegicus under different altitudes. Compared to R. norvegicus, the expression levels of all the tested cell cycle-related genes were significantly higher in M. baileyi. These results suggested that, the up-regulated p21 and down-regulated CyclinD1, CyclinE, CDK6 and CDK2 genes, which can induce the arrest of cell cycle G1 and thus provide sufficient time for DNA repair and ensure the accuracy of DNA replication, contribute to the long-time adaptation of M. baileyi to hypoxic environments. The regulation of cell cycle in the liver of M. baileyi was not only related to the expression levels of cell cycle-related genes, but may also be related to the variation sites at residue 27 and 105 of p21 and CyclinB1, respectively.
2020,39(1): 1-14 收稿日期:2018-11-21
分类号:Q959.837;Q344+.13
基金项目:青海省自然科学基金项目(2016-ZJ-901)
作者简介:安志芳(1990-),博士研究生,主要从事高原动物资源保护与利用研究,E-mail:anzhifang90@126.com
*通信作者:魏登邦,E-mail:weidengbang@163.com
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