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Issue:ISSN 1000-7083
          CN 51-1193/Q
Director:Sichuan Association for Science and Technology
Sponsored by:Sichuan Society of Zoologists; Chengdu Giant Panda Breeding Research Foundation; Sichuan Association of Wildlife Conservation; Sichuan University
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Your Position :Home->Past Journals Catalog->2019 Vol.38 No.6

Research on Quaternary Ammonium Compounds Disinfectant-Resistance of Escherichia coli in Raw Milk
Author of the article:GUO Lijuan1,2, SUN Fenghui1,2, ZHANG Yanjiao1,2, LIU Haifeng1,2, LIAO Xiaowei1,2, DAI Min1,2*
Author's Workplace:1. School of Laboratory Medicine, Chengdu Medical College, Chengdu 610500, China;
2. Sichuan Provincial Engineering Laboratory for Prevention and Control Technology of Veterinary Drug Residue in Animal-Origin Food, Chengdu Medical College, Chengdu 610500, China
Key Words:Escherichia coli; quaternary ammonium compounds; disinfectant; resistance
Abstract:Objective To investigate the contamination status of Escherichia coli in raw milk and their resistance to common quaternary ammonium compounds (QACs) disinfectants. Methods The minimal inhibitory concentration (MIC) values of 4 QACs disinfectants (cetyltrimethyl ammonium bromide, CTAB; benzalkonium chloride, BC; didecyldimethyl ammonium chloride, DDAC; cetyl pyridine chloride, CPC) for 45 E. coli isolates from raw milk were determined by agar dilution method, and 10 resistance genes were detected by PCR. The correlation between MIC values and resistance genes was analyzed also. Results E. coli showed different degrees of resistance to the 4 disinfectants. The MIC values of the 4 disinfectants tested against E. coli were larger than the standard strain, and the ratio was BC=DDAC > CTAB > CPC. The de tection rate of resistance genes to QACs disinfectants was 4.44%-84.44%:ydgF(84.44%) > ydgE(80.00%) > sugE(c) (66.67%) > emrE(40.00%) > mdfA(37.78%) > qacEΔ1(33.33%) > qacE(17.78%) > qacF(13.33%) > qacG(11.11%) > sugE(p)(4.44%). There is a significant difference between the detection rate of qacF gene and CPC of 128 mg·L-1 concentration. Conclusion E. coli with more anti-disinfectant gene combinations and higher MIC values are prevalent in the milk from the tested farm. Therefore, the resistance of E. coli to disinfectants should be taken seriously.
2019,38(6): 623-629,645 收稿日期:2019-07-26
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