Latest Cover

Online Office

Contact Us

Issue:ISSN 1000-7083
          CN 51-1193/Q
Director:Sichuan Association for Science and Technology
Sponsored by:Sichuan Society of Zoologists; Chengdu Giant Panda Breeding Research Foundation; Sichuan Association of Wildlife Conservation; Sichuan University
Address:College of Life Sciences, Sichuan University, No.29, Wangjiang Road, Chengdu, Sichuan Province, 610064, China
Fax:+86-28-85410485 &
Your Position :Home->Past Journals Catalog->2019 Vol.38 No.3

Spermatophore Characters and Effects of pH and Temperature on Sperm Motility of Poecilia latipinna
Author of the article:LI Jitong1,2, YANG Lei3, WANG Jing3, WANG Bing3, YANG Weili2, LI Ruijing2, ZHANG Yongzhong3*
Author's Workplace:1. Department of Nephrology and Rheumatology, Affiliated Children's Hospital of Zhengzhou University, Zhengzhou 450018, China;
2. Henan Provincial Key Laboratory of Children's Genetics and Metabolic Diseases, Zhengzhou 450018, China;
3. Department of Life Sciences, Liaocheng University, Liaocheng, Shandong Province 252059, China
Key Words:Poecilia latipinna; spermatophore; sperm motility; pH; temperature
Abstract:By using Poecilia latipinna as experimental material, the process of spermatophore breakdown and the factors affecting the process, sperm motility and the factors influencing sperm motility were studied in this study. We found that the spermatophore began to break at about 5 min and completely ruptured at about 12 min after the semen was diluted with Hank's balanced salt solution (HBSS). In addition, we also observed that all the sperms released from the broken spermatophore were temporarily dormant, and the sperms in HBSS diluent were activated after about 50 min. The percentage of swimming sperm, swimming duration and velocity of the sperm were also studied by a computer-assisted semen analyser in HBSS at different pH and temperatures. The results showed that neutral and weak alkaline solutions with pH from 7 to 8 were optimum for sperm swimming (or motility), while acidic solutions with pH<7 or strong alkaline solutions with pH>9 both reduced sperm motility. The total motility span of sperm was significantly longer, and the sperm swimming velocity was significantly slower when kept at low temperature (4℃) compared with those at room temperature (20℃). Therefore, these findings provide valuable references for artificial insemination, and for the reproductive biological knowledge of these fish.
2019,38(3): 311-318 收稿日期:2019-01-22
郭明申, 刘龙, 穆淑梅, 等. 2006. 孔雀鱼精子发生的显微与超微结构[J]. 河北大学学报, 26(6):653-658.
李纪同, 王晶, 王冰, 等. 2012. 环境pH及渗透压对玫瑰无须鲃精子运动的影响[J]. 四川动物, 31(3):353-357.
Alavi SMH, Cosson J, Karami M, et al. 2004. Spermatozoa motility in the Persian sturgeon, Acipenser persicus:effects of pH, dilution rate, ions and osmolality[J]. Reproduction, 128(6):819-828.
Dietrich GJ, Kowalski R, Wojtczak M, et al. 2005. Motility parameters of rainbow trout (Oncorhynchus mykiss) spermatozoa in relation to sequential collection of milt, time of post-mortem storage and anesthesia[J]. Fish Physiology and Biochemistry, 31(1):1-9.
Dong Q, Huang C, Tiersch TR. 2006. Post-thaw amendment of cryopreserved sperm for use in artificial insemination of a viviparous fish, the green swordtail Xiphophorus helleri[J]. Aquaculture, 259(1-4):403-414.
Dzyuba BB, van Look KJ, Kholodnyy VS, et al. 2008. Variable sperm size and motility activation in the pipefish, Syngnathus abaster; adaptations to paternal care or environmental plasticity?[J]. Reproduction Fertility & Development, 20(4):474-482.
Gardiner DM. 1978. Utilisation of extracellular glucose by spermatozoa of two viviparous fishes[J]. Comparative Biochemistry and Physiology:Part A, Molecular & Integrative Physiology, 59(2):165-168.
Huang C, Dong Q, Walter RB, et al. 2004. Initial studies on sperm cryopreservation of a live-bearing fish, the green swordtail Xiphophorus helleri[J]. Theriogenology, 62(1-2):179-194.
Huang CJ, Dong QX, Tiersch TR. 2004. Sperm cryopreservation of a live-bearing fish, the platyfish Xiphophorus couchianus[J]. Theriogenology, 62(6):971-989.
Kallman KD. 1975. The platyfish, Xiphophorus maculates[M]//King RC. Handbook of genetics. New York:Plenum Publishing Corporation.
Levanduski MJ, Cloud JG. 1988. Rainbow trout (Salmo gairdneri) semen:effect of non-motile sperm on fertility[J]. Aquaculture, 75(1-2):171-179.
Leach B, Montgomerie R. 2000. Sperm characteristics associated with different male reproductive tactics in bluegills (Lepomis macrochirus)[J]. Behavioral Ecology and Sociobiology, 49(1):31-37.
Paniagua-Chavez CG, Tiersch TR. 2001. Laboratory studies of cryopreservation of sperm and trochophore larvae of the eastern oyster[J]. Cryobiology, 43(3):211-223.
Stoss J. 1983. Fish gamete preservation and spermatozoan physiology[M]//Hoar WS, Randall DJ, Donaldson EM. Fish physiology, behavior and fertility control. San Diego:Academic Press.
Tanaka H, Oka Y. 2005. Chaotropic ions and multivalent ions activate sperm in the viviparous fish guppy Poecilia reticulata[J]. Biochimica et Biophysica Acta-General Subjects, 1724(1-2):173-180.
Wilson-Leedy JG, Kanuga MK, Ingermann RL. 2009. Influence of osmolality and ions on the activation and characteristics of zebrafish sperm motility[J]. Theriogenology, 71(7):1054-1062.
Wojtczak M, Dietrich GJ, Slowinska M, et al. 2007. Ovarian fluid pH enhances motility parameters of rainbow trout (Oncorhynchus mykiss) spermatozoa[J]. Aquaculture, 270(1-4):259-264.
Yang H, Hazlewood L, Walter RB, et al. 2006. Effect of osmotic immobilization on refrigerated storage and cryopreservation of sperm from a viviparous fish, the green swordtail Xiphophorus helleri[J]. Cryobiology, 52(2):209-218.
Yang H, Tiersch TR. 2009. Sperm motility initiation and duration in a euryhaline fish, medaka (Oryzias latipes)[J]. Theriogenology, 72(3):386-392.
CopyRight©2021 Editorial Office of Sichuan Journal of Zoology 蜀ICP备08107403号-3