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Your Position :Home->Past Journals Catalog->2019 Vol.38 No.3

Spermatophore Characters and Effects of pH and Temperature on Sperm Motility of Poecilia latipinna
Author of the article:LI Jitong1,2, YANG Lei3, WANG Jing3, WANG Bing3, YANG Weili2, LI Ruijing2, ZHANG Yongzhong3*
Author's Workplace:1. Department of Nephrology and Rheumatology, Affiliated Children's Hospital of Zhengzhou University, Zhengzhou 450018, China;
2. Henan Provincial Key Laboratory of Children's Genetics and Metabolic Diseases, Zhengzhou 450018, China;
3. Department of Life Sciences, Liaocheng University, Liaocheng, Shandong Province 252059, China
Key Words:Poecilia latipinna; spermatophore; sperm motility; pH; temperature
Abstract:By using Poecilia latipinna as experimental material, the process of spermatophore breakdown and the factors affecting the process, sperm motility and the factors influencing sperm motility were studied in this study. We found that the spermatophore began to break at about 5 min and completely ruptured at about 12 min after the semen was diluted with Hank's balanced salt solution (HBSS). In addition, we also observed that all the sperms released from the broken spermatophore were temporarily dormant, and the sperms in HBSS diluent were activated after about 50 min. The percentage of swimming sperm, swimming duration and velocity of the sperm were also studied by a computer-assisted semen analyser in HBSS at different pH and temperatures. The results showed that neutral and weak alkaline solutions with pH from 7 to 8 were optimum for sperm swimming (or motility), while acidic solutions with pH<7 or strong alkaline solutions with pH>9 both reduced sperm motility. The total motility span of sperm was significantly longer, and the sperm swimming velocity was significantly slower when kept at low temperature (4℃) compared with those at room temperature (20℃). Therefore, these findings provide valuable references for artificial insemination, and for the reproductive biological knowledge of these fish.
2019,38(3): 311-318 收稿日期:2019-01-22
分类号:Q959.4;Q955
基金项目:国家自然科学基金项目(31071268);河南省重点研发与推广专项(192102310074)
作者简介:李纪同(1982-),男,博士,研究方向:从事生殖和发育生物学研究,E-mail:ljtzch@yeah.net
*通信作者:张永忠,E-mail:Dr.yzz@126.com
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