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Issue:ISSN 1000-7083
          CN 51-1193/Q
Director:Sichuan Association for Science and Technology
Sponsored by:Sichuan Society of Zoologists; Chengdu Giant Panda Breeding Research Foundation; Sichuan Association of Wildlife Conservation; Sichuan University
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Your Position :Home->Past Journals Catalog->2019 Vol.38 No.3

Gene Structure, Sequence and Tissue Expression of Chicken G Protein-Coupled Receptor 85 Gene
Author of the article:SONG Liang, ZHANG Jiannan, LIU Haikun, MO Chunheng, LI Juan, WANG Yajun*
Author's Workplace:Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu 610065, China
Key Words:chicken; GPR85; G protein-coupled receptors; clone; tissue expression
Abstract:G protein-coupled receptor (GPCR) is a kind of membrane receptor superfamily. It is an important receptor on cell transmembrane to transmit signals and regulates many important physiological processes. Because the endogenous ligands for some GPCRs have not been identified, these GPCRs are still classified as orphan receptor. G protein-coupled receptor 85 (GPR85) is a member of orphan receptor in GPCR superfamily. Up till the present, the information regarding on the structure and expression of GPR85 gene in non-mammalian vertebrates is limited. In this study, by using RT-PCR and RACE PCR, we cloned the full cDNA sequence of GPR85 gene from chicken brain and revealed its gene structure. Furthermore, by using quantitative real-time PCR(qPCR), we also examined the mRNA expression of GPR85 gene in various chicken tissues. The results showed that the chicken GPR85 gene locates on chromosome 1 and consists of 2 exons. The coding region is located on exon 2. Chicken GPR85 gene has an open reading frame of 1 113 bp and encodes a seven-transmembrane receptor. Chicken GPR85 shows extremely high amino acid sequence identity (>93%) with that of Homo sapiens, Mus musculus, Rattus norvegicus, Xenopus tropicalis and Danio rerio. The result of qPCR revealed that chicken GPR85 gene is highly expressed in the brain region, anterior pituitary, adrenal glands and testis, but weakly expressed in other remaining tissues examined. To our knowledge, this study represents the first report concerning the gene structure and expression of GPR85 in chicken and lays the foundation for further exploring the physiological roles of GPR85 in chickens.
2019,38(3): 271-278 收稿日期:2018-04-29
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