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Issue:ISSN 1000-7083
          CN 51-1193/Q
Director:Sichuan Association for Science and Technology
Sponsored by:Sichuan Society of Zoologists; Chengdu Giant Panda Breeding Research Foundation; Sichuan Association of Wildlife Conservation; Sichuan University
Address:College of Life Sciences, Sichuan University, No.29, Wangjiang Road, Chengdu, Sichuan Province, 610064, China
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Your Position :Home->Past Journals Catalog->2019 Vol.38 No.3

Genetic Diversity of Invasive Species Rhinogobius cliffordpopei at the Source of the Pearl River
Author of the article:YAN Yuehui1,2, DING Xuemei1,2, LI Qiang2, LI Xu1*, TANG Lizhou2*
Author's Workplace:1. Southwest Forestry University, Kunming 650224, China;
2. Center for Yunnan Plateau Biological Resources Protection and Utilization, Qujing Normal University, Qujing, Yunnan Province 655011, China
Key Words:Rhinogobius cliffordpopei; cytochrome b gene; genetic diversity; genetic differentiation; the source of the Pearl River
Abstract:In order to understand the distribution characteristics of genetic diversity and the influencing factors of the invasive fish Rhinogobius cliffordpopei at the source of the Pearl River, the genetic diversity and genetic differentiation of 9 natural reservoir populations at the source of the Pearl River, were analyzed using mitochondrial cytochrome b (cyt b) gene as molecular marker. The complete sequence of cyt b gene was 1 141 bp, including 1 072 invariable sites and 69 variable sites, without insertion and deletion sites. Five haplotypes were identified from 96 individuals of this species. The population haplotype diversity and the nucleotide diversity were 0.359±0.059 and 0.021±0.010, respectively. These data indicated that this species formed low haplotype diversity and high nucleotide diversity. The results of rooted phylogenetic trees and the median-joining network relationship showed that all haplotypes of the R. cliffordpopei were separated from the outgroup (R. giurinus, R. brunneus and R. rubromaculatus), forming a monophyly and divided into 2 distinct clades. The analysis of molecular variation showed that the genetic variation between populations (62.99%) of R. cliffordpopei was significantly higher than that within populations (37.01%). And the fixed index was 0.630 (P<0.01), which confirmed that the population of R. cliffordpopei had a significant genetic structure. All above results supported that the invasive species R. cliffordpopei had high genetic diversity and remarkable genetic structure at the source of the Pearl River, and these might be influenced by the founder event and genetic bottleneck effect. The multiple human introduction and dam isolation might provide conditions for individual distribution and mutation accumulation of R. cliffordpopei. These findings will provide scientific guidance for the prevention and control of invasive damage of R. cliffordpopei and the conservation of species diversity of indigenous fish species.
2019,38(3): 263-270 收稿日期:2018-12-10
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