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Issue:ISSN 1000-7083
          CN 51-1193/Q
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Your Position :Home->Past Journals Catalog->2019 Vol.38 No.2

Microstructure and Sub-Microstructure of the Keratinized Nuptial Spines of Male Leptobrachium boringii (Liu, 1945)
Author of the article:ZHANG Wuyuan1,2,3, LU Yuyan1,2,3, SHI Jingsong1,4,5, ZHU Lei6, LI Pipeng1,2,3*
Author's Workplace:1. Institute of Herpetology, Shenyang Normal University, Shenyang 110034, China;
2. Liaoning Key Lab of Biodiversity and Evolution, Shenyang Normal University, Shenyang 110034, China;
3. College of Life Science, Shenyang Normal University, Shenyang 110034, China;
4. Institute of Vertebrate Paleontology and Paleoanthropology, Chinese Academy of Sciences, Beijing 100044, China;
5. Key Laboratory of Vertebrate Evolution and Human Origins, Chinese Academy of Sciences, Beijing 100044, China;
6. Chengdu Bird Watching Society, Chengdu 610041, China
Key Words:Leptobrachium boringii; keratinized nuptial spines; skin; epidermal ridge
Abstract:The microstructure and sub-microstructure of the keratinized nuptial spines and their relative peripheral skin of male Leptobrachium boringii were studied by general histological and scanning electron microscope (SEM) methods. The results showed that the spine of L. boringii is the skin derivative which rises up as an inverted V-like conical protuberance from the periheral skin area. Spine is composed of the external part of keratinized epidermis and inner part of dermis. The spine epidermis is stratified squamous epithelium and can be divided into 4 layers, of which the outermost layer is keratinized without cell outline and stained deeply red in color. The fold of skin encircles around the spine base and extends upward to form the spine. The spine epidermal ridge extends into the dermis under the epithermis. To the best of the authors' knowledge, this structure has never been reported in other studied frogs and toads. Furthermore, the underlying loose connective tissue of the epidermis makes up the spine dermis, where no stratum compactum and stratum spongiosum can be distinguished. In spine dermis, no skin glands were found but only a small number of pigment cells and capillary vessels. Under SEM observation, the tip of the spines looks like a conical "hill", and the outline of superficial epidermal cell is distinguishable in the surface. The cells arrange in imbricate pattern. At the junction of spine and its peripheric skin, the superficial epithermal cells are polygonous and keritinized. Both the upper and lower surfaces of keratinized epithelial cells have an uneven pattern. The cells are connected to each other with anchoring junctions.
2019,38(2): 186-193 收稿日期:2018-09-28
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