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Issue:ISSN 1000-7083
          CN 51-1193/Q
Director:Sichuan Association for Science and Technology
Sponsored by:Sichuan Society of Zoologists; Chengdu Giant Panda Breeding Research Foundation; Sichuan Association of Wildlife Conservation; Sichuan University
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Your Position :Home->Past Journals Catalog->2019 Vol.38 No.2

Cloning and Polymorphism of Agouti Gene and Its Expression in Different Skin Color Phenotypes in Andrias davidianus
Author of the article:JIANG Wei, DENG Jie, WANG Qijun, ZHAO Hu, KONG Fei, ZHANG Hongxing*
Author's Workplace:Shaanxi Key Laboratory for Animal Conservation, Shaanxi Institute of Zoology, Xi'an 710032, China
Key Words:Andrias davidianus; body color; melanin; Agouti gene; expression
Abstract:Agouti protein is one of key regulatory factors involving in melanin biosynthesis of mammals and birds and plays a role in animal skin color formation. To investigate the relationship between Agouti gene and skin color formation of amphibians, the Agouti gene of giant salamander (Andrias davidianus) was cloned by PCR followed by sequence characterization using bioinformatics tools. Meanwhile, the expression patterns of Agouti gene in 10 different tissues and in 4 types of skin color phenotypes were detected by quantitative real-time PCR. Furthermore, nucleotide mutation sites of Agouti gene were explored by direct DNA sequencing. The obtained Agouti cDNA was 1 068 bp in length and contained a 399 bp open reading frame encoding a peptide of 132 amino acids. Sequence analysis predicted that Agouti of A. davidianus had a conserved Agouti domain. Multiple sequence alignment showed that Agouti of A. davidianus had high amino acids similarity with amphibians and reptiles, but had low similarity with mammals and birds. These results were further confirmed by phylogenetic tree analysis. Quantitative expression analysis showed that Agouti mRNA expressed in all the tested tissues of A. davidianus, and among of which, the maximum expression level was detected in skin. Besides, the expression of Agouti gene in yellow skin was significantly higher than that in other colors of skins. Polymorphism analysis revealed that the cDNA sequences of Agouti genes in A. davidianus were totally identical among different skin color phenotypes. The specific sequence characteristics and expression pattern of Agouti gene in A. davidianus suggested that there might be different regulatory mechanisms of this gene in skin color formation compared with other species. These findings provide helpful information for further studying the role of Agouti in skin color formation of A. davidianus.
2019,38(2): 121-129 收稿日期:2018-08-21
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