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Issue:ISSN 1000-7083
          CN 51-1193/Q
Director:Sichuan Association for Science and Technology
Sponsored by:Sichuan Society of Zoologists; Chengdu Giant Panda Breeding Research Foundation; Sichuan Association of Wildlife Conservation; Sichuan University
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Your Position :Home->Past Journals Catalog->2019 Vol.38 No.1

Preliminary Research of microRNA in Periplaneta americana
Author of the article:BAO Zheng1, YANG Mingyu2, ZHANG Xiuyue1, YUE Bisong1,2,3, FAN Zhenxin1*
Author's Workplace:1. Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu 610065, China;
2. Sichuan Engineering Research Center for Medicinal Animals, Xichang, Sichuan Province 615000, China;
3. Sichuan Key Laboratory for Medicinal American Cockroach, Chengdu 610031, China
Key Words:Periplaneta americana; microRNA; differential expression
Abstract:American cockroach (Periplaneta americana) is known as one of the public health pests worldwide. Meanwhile, its extracts are valuable for medical use. In this study, sequencing of microRNAs of the reared cockroaches was carried out. A total of 12 155 616 and 9 847 263 sequences were obtained from the male and female individuals, respectively. The length of sequences mainly ranged from 18 nt to 23 nt with a bimodal distribution at 22 nt and 29 nt. Annotation of these clean reads were performed according to databases of Rfam and NCBI by Blastn. Finally, 57 known microRNAs and 152 novel microRNAs were identified from the male group, while 53 known microRNAs and 94 novel microRNAs were identified from the female group. Differential expression analysis indicated that only one microRNA (miR-750) was significantly up-regulated in female P. americana compared to that of the male. This is the first study concerning on the composition and function of microRNAs in P. americana at genomic level, and thus lay a foundation for further study on P. americana.
2019,38(1): 47-55 收稿日期:2018-05-10
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