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Issue:ISSN 1000-7083
          CN 51-1193/Q
Director:Sichuan Association for Science and Technology
Sponsored by:Sichuan Society of Zoologists; Chengdu Giant Panda Breeding Research Foundation; Sichuan Association of Wildlife Conservation; Sichuan University
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Your Position :Home->Past Journals Catalog->2019 Vol.38 No.1

Cloning and Functional Analysis of Caspase-7 Gene from Dugesia japonica
Author of the article:WANG Zhihong, WANG Chao, PENG Rui*
Author's Workplace:Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu 610065, China
Key Words:Dugesia japonica; Caspase-7 gene; cloning; tissue remodeling
Abstract:In this study, the full length of the Caspase-7 gene of Dugesia japonica was first cloned using the rapid amplification of cDNA ends technique. The Caspase-7 gene of D. japonica is 935 bp in length, encoding 251 amino acids, and containing 86 bp and 93 bp of the 5'-UTR and 3'-UTR regions, respectively. It also contains 2 key conserved domains of Caspase family, LSHG and QAC(Q/R)G. The three-dimensional structure of Caspase-7 protein was predicted using SWISS-MODEL workspace. The result showed that Caspase-7 protein contains 2 catalytic units and the potential catalytic sites consist of 4 surface rings. The result of phylogenetic analysis reveals that planarian Caspase-7 gene has closer relationship with that of Hydra vulgaris than other species of Platyhelminthes. Quantitative real-time PCR technology was used to evaluate the expression of Caspase-7 gene at different times of planarian regeneration, and the result suggested that Caspase-7 gene might be involved in the regulation of apoptosis in planarian. In addition, knocking down the in vivo expression of Caspase-7 gene by feeding dsRNA interference in the planarian did not influence the regeneration of planarian, but led to a significantly decreased expression of Djclg-3 and PCNA gene, indicating a significantly reduced apoptosis and proliferation. Finally, the regenerative or untreated planarians were dissolving under the starvation condition, and this should be due to the down-regulated expression of Caspase-7, thereby implied that Caspase-7 gene plays a key role in planarian tissue remodeling.
2019,38(1): 11-19 收稿日期:2018-04-26
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