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Issue:ISSN 1000-7083
          CN 51-1193/Q
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Your Position :Home->Past Journals Catalog->2018 Vol.37 No.6

Effects of Periplaneta americana Powder on Blood Glucose and Creatinine in Mice
Author of the article:GAO Jie1, LIU Jinhua1, SUN Xiaohong1, LIU Shiyao1, SHEN Yongmei2, ZHANG Xiuyue1, YUE Bisong1*
Author's Workplace:1. Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu 610065, China;
2. Sichuan Key Laboratory of MedicinalPeriplaneta americana, Chengdu 610031, China
Key Words:Periplaneta americana; high-fat diet; blood glucose; creatinine
Abstract:Objective To investigate the effects of Periplaneta americana powder on the body mass, blood glucose and creatinine in mice fed with high-fat diet.Methods The mice fed with high-fat diet were divided into control and experimental groups (P. americana powder low dose group 100 mg·kg-1; medium dose group 200 mg·kg-1 and high dose group 400 mg·kg-1); and the body mass changes were observed. The serum samples were taken and the fasting blood glucose and creatinine levels were then measured; the mRNA expression levels of glucose-6-phosphatase (G-6-Pase) and glucose transporter 2 (GLUT2) in liver tissues and monocyte chemoattractant protein-1 (MCP-1) in kidney tissues were detected by real-time PCR at 42nd and 49th day.Results The body mass growth rates of the middle and high dose groups declined significantly after 42 and 49 days compared to the control (P<0.05, P<0.01). On the 42nd day, the content of blood glucose, creatinine and MCP-1 mRNA levels in mice of experimental groups showed the decreasing trends, but these differences were not significant (P>0.05); G-6-Pase mRNA levels in the middle and high dose groups were dramatically lower (P<0.01); GLUT2 mRNA levels did not change notably (P> 0.05). On the 49th day, the content of blood glucose and creatinine in the low and high dose groups were significantly lower than those of the control (P<0.05); G-6-Pase, GLUT2 and MCP-1 mRNA levels in the low, medium and high dose groups were remarkably decreased (P<0.05, P<0.01).Conclusion P. americana powder can reduce the body mass growth rate, the content of blood glucose and creatinine in mice fed with high-fat diet. Decreasing the expression of G-6-Pase mRNA and regulating the expression of GLUT2 mRNA in liver may be one of the mechanisms of P. americana powder to decrease the blood glucose level. Moreover, P. americana powder may reduce the renal injury by declining the expression of MCP-1 mRNA in kidney.
2018,37(6): 667-671 收稿日期:2018-07-09
分类号:Q95-33
作者简介:高洁,女,硕士研究生,研究方向:动物分子生物学
*通信作者:岳碧松,E-mail:bsyue@scu.edu.cn
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