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Issue:ISSN 1000-7083
          CN 51-1193/Q
Director:Sichuan Association for Science and Technology
Sponsored by:Sichuan Society of Zoologists; Chengdu Giant Panda Breeding Research Foundation; Sichuan Association of Wildlife Conservation; Sichuan University
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Your Position :Home->Past Journals Catalog->2018 Vol.37 No.5

Comparison of Gene Expression of Toll-Like Receptors and Antimicrobial Peptides in the Immune Organs and Tissues of Wujin Pig, Qingyu Pig and Chenghua Pig
Author of the article:ZHANG Jingmei1#, GU Yiren2#, LI Jiangling2, CHEN Xiaohui2, ZENG Kai2, LYU Xuebin2*, GAO Rong1*
Author's Workplace:1. Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, Key Laboratory of Animal Disease Prevention and Food Safety of Sichuan Province, College of Life Sciences, Sichuan University, Chengdu 610065, China;
2. Sichuan Academy of Animal Science, Chengdu 610066, China
Key Words:pig; toll-like receptors gene; antimicrobial peptides; gene expression; immune organs and tissues
Abstract:Toll-like receptors (TLRs) and antimicrobial peptides are important components of the host natural immune system and are an important barrier to prevent harmful microorganisms or viruses. Wujin pig, Qingyu pig and Chenghua pig are the well-known Chinese unique local subspecies of pig breed, and they own the merits of high fertility and strong disease resistance. In this study, the innate immunity characteristics of 8-month-old Wujin pigs, Qingyu pigs, Chenghua pigs and Yorkshire pigs were compared by using the local immune tissues and systemic immune organs. Real-time fluorescent quantitative PCR was used to detect the expression levels of TLRs (TLR1, TLR2, TLR4, TLR7 and TLR9) and antimicrobial peptide-encoding genes (pBD-1 and PR-39) in thymus, spleen, amygdala, mesenteric lymph node and hilar lymph nodes. The results showed that compared to the control pigs, the expression levels of TLR7 and PR-39 in the thymus, TLR4 and TLR7 in the spleen, TLR4 and TLR9 in the mesenteric lymph nodes, and TLR1, TLR4, TLR7 and TLR9 in the hilar lymph nodes were significantly higher (P<0.05) in Chenghua pigs; the expression levels of TLR1 and TLR9 in the thymus, TLR1, TLR2, TLR4, TLR7, TLR9 and pBD-1 in the tonsils, and TLR7 in the mesenteric lymph nodes of Wujin pig were significantly higher than those in other pig breeds (P<0.05); the expression of PR-39 gene in the tonsils and mesenteric lymph nodes of Qingyu pigs was significantly higher than those in other pig breeds (P<0.05). These results provide useful clues for further screening the disease-resistant molecular markers of local pig hybrid breeding.
2018,37(5): 525-532 收稿日期:2018-03-13
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