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Issue:ISSN 1000-7083
          CN 51-1193/Q
Director:Sichuan Association for Science and Technology
Sponsored by:Sichuan Society of Zoologists; Chengdu Giant Panda Breeding Research Foundation; Sichuan Association of Wildlife Conservation; Sichuan University
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Your Position :Home->Past Journals Catalog->2018 Vol.37 No.5

Single Nucleotide Polymorphisms of the Partial Sequence of SLA-DRB Gene in Pujiang Population of Tibetan Pigs and Correlation with Growth Traits
Author of the article:ZHU Wei1, ZHU Lei2, HE Fan3, FU Lei1, SHUAI Surong3, JIANG Jianping1*
Author's Workplace:1. Chinese Academy of Sciences, Chengdu Institute of Biology, Chengdu 610041, China;
2. Sichuan Zhongce Environmental Technology Co., Ltd., Chengdu 611731, China;
3. College of Animal Science, Sichuan Agricultural University, Chengdu 611130, China
Key Words:Tibetan pig; SLA-DRB gene; cannon bone circumference; immune function; genetic breeding
Abstract:The single nucleotide polymorphism (SNP) of swine leukocyte antigen (SLA) gene is associated with the growth and development traits of swine. In this study, the partial sequence of SLA-DRB gene of 89 Aba Tibetan pigs (Pujiang county, Sichuan province) was sequenced to screen the stable markers for genetic breeding, and 14 loci of SNPs were identified. Based on the linkage relationships, these 14 loci of SNPs were allocated into 3 groups (loci group 1:g.11470, g.11945, g.11997, g.11387, g.11369 and g.11829; loci group 2:g.11532 and g.11838; loci group 3:g.11911 and g.11708) and 4 single polymorphic loci (g.11468, g.11440, g.11478 and g.11944). Correlation analyses were conducted between the genotypes of these loci or loci groups and 14 growth traits (i.e. body mass and body length). The polymorphism of loci group 3 was significantly associated with 11 growth traits (FDR<0.05), including withers height, body length and chest circumference, and the individuals with genotype TT/CG had smaller somatotype than that of others. This suggested that SLA-DRB gene might be a stable genetic marker for the miniaturization selection of Tibetan pigs. The cannon bone circumference (CBC) and ratios of CBC to other growth traits (except body mass), were tightly associated with the polymorphism of loci group 1, loci group 2, g.11468 and g.11478 (FDR<0.001). The individuals with genotypes GGGAAC/GGGAAC at loci group 1, GT/GT at loci group 2, C/C at g.11468 and C/C at g.11478 tend to have large CBC and CBC ratios. Given that the cannon bone marrow is a major site for the immune function of SLA genes, our results here provide theoretical basis and stable SNP loci for CBC based swine selective breeding and disease resistance oriented genetic breeding.
2018,37(5): 497-506 收稿日期:2018-03-27
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