Latest Cover

Online Office

Contact Us

Issue:ISSN 1000-7083
          CN 51-1193/Q
Director:Sichuan Association for Science and Technology
Sponsored by:Sichuan Society of Zoologists; Chengdu Giant Panda Breeding Research Foundation; Sichuan Association of Wildlife Conservation; Sichuan University
Address:College of Life Sciences, Sichuan University, No.29, Wangjiang Road, Chengdu, Sichuan Province, 610064, China
Fax:+86-28-85410485 &
Your Position :Home->Past Journals Catalog->2018 Vol.37 No.4

Application of DNA Barcoding in Identification of Encyrtid Parasitoids of Acanthococcus (=Eriococcus) lagerstroemiae
Author of the article:ZHANG Zeyuan1, QIN Yaoguang2, LI Shuqiang2*
Author's Workplace:1. Division of the High School Affiliated to Renmin University of China, Beijing 100080, China;
2. Key Laboratory of Zoological Systematics and Evolution, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China
Key Words:encyrtid parasitoids; mitochondrial COⅠ gene; species identification; Acanthococcus (=Eriococcus) lagerstroemiae
Abstract:Acanthococcus (=Eriococcus) lagerstroemiae is an important pest of Punica granatum L. and Lagerstroemia indica L. and broadly distributed in China. Encyrtid parasitoids play a key role in the control of A. (=Eriococcus) lagerstroemiae. We obtained samples of the encyrtid parasitoids by rearing them from nymphs of A. (=Eriococcus) lagerstroemiae collected from different areas of China. DNA barcoding approach was applied to the identification of encyrtid parasitoids and followed by a preliminary morphological identification. DNA sequence data of the mitochondrial COⅠ gene were generated from 93 individuals of encyrtid parasitoids. The results showed that the inter-specific COⅠ divergence was 0.073 2~0.186 4 with an average of 0.111 0; and the intra-specific COⅠ variation was 0~0.026 5 with an average of 0.007 4. The inter-specific COⅠ divergence was greatly larger than intra-specific COⅠ variation in the encyrtid parasitoid species. Our results indicated that, as a complementary approach to morphology, DNA barcoding can help us to rapidly and accurately identify the encyrtid species.
2018,37(4): 406-414 收稿日期:2017-09-21
贺冬英, 程建, 赵胡, 等. 2008. 紫薇绒蚧的生物学特性与药剂防效[J]. 昆虫知识, 45(5):811-814.
姜年俊, 许惠. 1998. 紫薇绒蚧的观察研究[J]. 安徽农业大学学报, 25(2):44-46.
焦浩, 张玉俊. 2011. 宝鸡地区紫薇绒蚧的发生规律与防治策略[J]. 陕西农业科学, (1):107-108.
马金华. 2011. 四川攀西地区榴绒蚧的发生与生物学特性研究[J]. 中国南方果树, 40(5):12-14.
宋南, 刘杰, 彩万志, 等. 2013. DNA条形码在昆虫分类中的应用[J]. 四川动物, 32(3):470-474.
肖金花, 肖晖, 黄大卫. 2004. 生物分类学的新动向——DNA条形编码[J]. 动物学报, 50(5):852-855.
徐志宏, 黄建. 2004. 中国介壳虫寄生蜂志[M]. 上海:上海科学技术出版社.
张合彩, 乔格侠. 2008. 五节根蚜亚科(半翅目:蚜总科:瘿绵蚜科)基于线粒体基因COⅡ的分子系统学研究[J]. 四川动物, 27(5):754-757.
张钦书, 孟庆喜. 1990. 石榴绒蚜生物学特性及防治研究初报[J]. 莱阳农学院学报, 7(2):145-146.
赵文娟, 徐清芳, 邢治英, 等. 2016. 临沂大学校园园林植物害虫调查初报[J]. 中国园艺文摘, 32(1):99-100.
赵洋民, 赵雪艳, 韩邦义, 等. 1997.紫薇绒蚧化学防治试验[J]. 山东林业科技, 113:20-22.
Bickford D, Lohman DJ, Sodhi NS, et al. 2007. Cryptic species as a window on diversity and conservation[J]. Trends in Ecology & Evolution, 22:148-155.
Chesters D, Wang Y, Yu F, et al. 2012. The integrative taxonomic approach reveals host specific species in an encyrtid parasitoid species complex[J]. PLoS ONE, 7(5):e37655. DOI:10.1371/journal.pone.0037655.
Hall TA. 1999. BioEdit:a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT[J]. Nucleic Acids Symposium Series, 41:95-98.
Hebert PD, Cywinska A, Ball SL, et al. 2003. Biological identifications through DNA barcodes[J]. Proceedings of the Royal Society of London B:Biological Sciences, 270(1512):313-321.
Merchant ME, Gu M, Robbins J, et al. 2014. Discovery and spread of Eriococcus lagerstroemiae Kuwana (Hemiptera:Eriococcidae), a new invasive pest of crape myrtle, Lagerstroemia spp.[J/OL]. (2014-03-16).
Pasquer F, Pfunder M, Frey B, et al. 2009. Microarray-based genetic identification of beneficial organisms as a new tool for quality control of laboratory cultures[J]. Biocontrol Science & Technology, 19(8):809-833.
Smith MA, Rodriguez JJ, Whitfield JB, et al. 2008. Extreme diversity of tropical parasitoid wasps exposed by iterative integration of natural history, DNA barcoding, morphology, and collections[J]. Proceedings of the National Academy of Sciences, 105:12359-12364.
Tamura K, Dudley J, Nei M, et al. 2007. MEGA4:molecular evolutionary genetics analysis (MEGA) software version 4.0[J]. Molecular Biology and Evolution, 24:1596-1599.
Thompson JD, Higgins DG, Gibson TJ. 1994. CLUSTAL W:improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice[J]. Nucleic Acids Research, 22(22):4673-4680.
Wang Y, Li CD, Zhang YZ. 2014. A taxonomic study of Chinese species of the insidiosus group of Metaphycus (Hymenoptera:Encyrtidae)[J]. ZooKeys, 406:53-88.
Wang Z, Chen Y, Gu M, et al. 2016. Crapemyrtle bark scale:a new threat for crapemyrtles, a popular landscape plant in the U.S.[J]. Insects, 7(4):78. DOI:10.3390/insects 7040078.
Zhang AB, Hao MD, Yang CQ, et al. 2017. BarcodingR:an integrated R package for species identification using DNA barcodes[J]. Methods in Ecology and Evolution, 8:627-634.
Zhang YZ, Huang DW. 2004. A review and an illustrated key to genera of Encyrtidae (Hymenoptera:Chalcidoidea) from China[M]. Beijing:Science Press:166.
Zhang YZ, Si SL, Zheng JT, et al. 2011. DNA barcoding of endoparasitoid wasps in the genus Anicetus reveals high levels of host specificity (Hymenoptera:Encyrtidae)[J]. Biological Control, 58(3):182-191.
Zhou MJ, Xiao JH, Bian SN, et al. 2012. Molecular approaches identify known species, reveal cryptic species and verify host specificity of Chinese Philotrypesis (Hymenoptera:Pteromalidae)[J]. Molecular Ecology Resources, 12(4):598-606.
Zhou QS, Polaszek A, Wang Y, et al. 2018. Parasitoid-host associations of the genus Coccophagus (Hymenoptera:Aphelinidae) in China[J]. Zoological Journal of the Linnean Society, 182:38-49.
Zhou QS, Xi YQ, Yu F, et al. 2014. Application of DNA barcoding to the identification of Hymenoptera parasitoids from the soybean aphid (Aphis glycines) in China[J]. Insect Science, 21(3):363-373.
CopyRight©2018 Editorial Office of Sichuan Journal of Zoology